A Liposome Immunoassay Based on a Chemiluminescense Reaction
A Liposome Immunoassay Based on a Chemiluminescense Reaction
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基于化学发光反应的脂质体免疫分析
DOI：
10.1248/cpb.37.1629
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发表时间：
1989
影响因子：
1.7
通讯作者：
Yasuo Suzuki
中科院分区：
文献类型：
--
作者：
Tohru Nakamura;S. Hoshino;N. Hazemoto;M. Haga;Y. Kato;Yasuo Suzuki
报道了一种新的、灵敏的免疫测定法，涉及脂质体免疫测定法与化学发光检测的组合。用溶细胞素-半抗原缀合物裂解脂质体，释放包埋的葡萄糖氧化酶。通过光纤和光电倍增管监测酶促形成的过氧化氢和异鲁米诺反应产生的化学发光。在没有结合/游离分离程序的情况下，通过脂质体裂解和酶反应对免疫反应进行化学放大，可以检测到 1.7 ng/分析管的地高辛（模型分析物）。当使用脂质体包被的微孔板将完整的脂质体与释放的酶分离时，检测的灵敏度提高至 165 pg/测定管。
A new, sensitive immunoassay involving the combination of a liposome immunoassay with chemilluminescence detection is reported. Lysis of the liposomes with cytolysin-hapten conjugates released entrapped glucose oxidase. Chemiluminescence produced by reaction of the enzymatically formed hydrogen peroxide and isoluminol was monitored via an optical fiber and photomultiplier. Without a bound/free separation procedure, this chemical amplification of the immune reaction by liposome lysis and enzymatic reaction allowed detection of 1.7 ng/assay tube of digoxin, the model analyte. When liposome-coated microplates were used to separate intact liposomes from released enzyme, the sensitivity of detection was improved to 165 pg/assay tube.