Analysis of genetic diversity in cytoplasmic male sterility, and association of mitochondrial genes with petaloid-type cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee)
Analysis of genetic diversity in cytoplasmic male sterility, and association of mitochondrial genes with petaloid-type cytoplasmic male sterility in tuber mustard (Brassica juncea var. tumida Tsen et Lee)
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DOI：
10.1007/s11033-009-9830-x
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发表时间：
2010-02
影响因子：
2.8
通讯作者：
Xiaolin Yu;H. Lu;G. Lu;Zhu-jun Chen;Jiashu Cao;Y. Hirata
中科院分区：
文献类型：
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作者：
Xiaolin Yu;H. Lu;G. Lu;Zhu-jun Chen;Jiashu Cao;Y. Hirata
本研究采用远缘杂交和回交的方法，选育出了稳定的榨菜细胞质雄性不育系。在这种CMS植物中，除了花药外，所有花器官都是正常的，花药转化为花瓣或管状结构。最近，已克隆了两个在榨菜细胞质雄性不育系中特异存在的线粒体基因atpA和dorf 220，并对其进行了部分鉴定。本研究以7种油菜和萝卜属作物（包括5个不育系及其相应的保持系）为材料，比较了它们线粒体基因组中蛋白质编码基因的组成。在43个线粒体基因中，atpA、orf 220、orf 256和orf 305/orf 324等4个基因在榨菜不育系和保持系间存在多态性。聚类分析结果表明，榨菜瓣状CMS是一种新的CMS类型，在系统发育水平上与甘蓝型油菜的瓣状CMS更为接近。这些基因的单个扩增的结果表明，存在4个序列特征扩增区（SCAR）标记，这使得快速和可靠的鉴定该CMS。theorf 220和orf 256基因仅在不育系中表达，而theorf 305基因在保持系中表达。不同器官特异性标记基因的不同表达模式表明，在榨菜器官/组织发育过程中，线粒体蛋白质的数量受到差异调节。本研究结果表明，上述4个线粒体基因与榨菜瓣状细胞质雄性不育表型相关。
In our previous study, we bred a stable cytoplasmic male sterility (CMS) line of tuber mustard by using distant hybridization and subsequent backcrosses. In this CMS plants, all floral organs are normal except the anthers, which are transformed into petals or tubular structures. Recently, 2 mitochondrial genes—atpAandorf220—that are distinctively present in the CMS line of tuber mustard were cloned and partially characterized. In our study of genetic diversity analysis of CMS, 7 species ofBrassicaandRaphanuscrops, which included 5 CMS lines and their respective maintainer lines, were used to compare the constitution of protein-coding genes in the mitochondrial genomes. In 4 of the 43 mitochondrial genes, namely,atpA,orf220,orf256,andorf305/orf324, polymorphisms were detected among the tuber mustard CMS line and its maintainer line. The results of a cluster analysis indicate that petaloid CMS phenotype of tuber mustard is a novel CMS type and is nearer to thenapCMS inBrassica napusat the phylogenetic level. The results of individual amplifications of these genes indicate the presence of 4 sequence-characterized amplified region (SCAR) markers, which enable rapid and reliable identification of this CMS. Expressions of theorf220andorf256genes were detected only in the CMS line, while expression of theorf305gene was detected in the maintainer line. The different expression patterns of different mitochondrial-specific marker genes indicate that the quantity of mitochondrial proteins is differentially regulated during organ/tissue development in tuber mustard. The results of this study suggest that the above mentioned 4 mitochondrial genes are associated with the petaloid CMS phenotype in tuber mustard.