Mapping of sex-linked genes onto the genome sequence using various aberrations of the Z chromosome in Bombyx mori
Mapping of sex-linked genes onto the genome sequence using various aberrations of the Z chromosome in Bombyx mori
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DOI：
10.1016/j.ibmb.2008.03.004
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发表时间：
2008-12-01
影响因子：
3.8
通讯作者：
Shimada, Toru
中科院分区：
文献类型：
--
作者：
Fujii, Tsuguru;Abe, Hiroaki;Shimada, Toru
家蚕的许多品系携带染色体畸变，它们是整合表型和基因组序列的有用资源。我们比较了三种Z染色体的分子结构，两个菌株具有染色体缺失，一个菌株具有涉及Z染色体的易位。利用聚合酶链反应标记，我们发现：（1）Z1染色体缺失超过6 Mb，包括近端;（2）Z（Vg）染色体在间质部分缺失1.5 Mb;（3）+（od）p（Sa）+W-p携带围绕+（od）基因的0.6 Mb Z衍生片段。精确地确定了这些缺失和易位的断点连接。通过缺失作图，我们缩小了不同油性（od），残留（Vg）和肌肉营养不良（Md）的区域，并确定了一个候选基因的广告。在ad突变体中检测到BmBLOS 2（与人类“溶酶体相关细胞器复合体的生物发生-1，亚基2”同源的Bombyx基因）中逆转录酶介导的缺失。虽然负责Vg和Md的基因没有明确确定，我们提出了候选基因的基础上，他们的位置和表型。(C)2008爱思唯尔有限公司保留所有权利。
Many strains of Bombyx mori carry chromosomal aberrations, and they are useful resources for integration between phenotypes and genomic sequences. We compared the molecular structures of three kinds of Z chromosomes, i.e., two strains with chromosome deletions and one strain with translocation involving the Z chromosome. Using polymerase chain reaction markers, we showed that: (1) the Z, chromosome lacks more than 6 Mb, including the proximal end; (2) the Z(Vg) chromosome lacks 1.5 Mb in the interstitial portion; and (3) the +(od)p(Sa)+W-p carries a 0.6-Mb Z-derived fragment surrounding the +(od) gene. The breakpoint junctions of these deletions and a translocation were precisely determined. Through deletion mapping, we narrowed down the regions where distinct oily (od), vestigial (Vg), and muscle dystrophy (Md) are located and identified a candidate gene for ad. A retroposon-mediated deletion in BmBLOS2-the Bombyx gene homologous to human "biogenesis of lysosome-related organelles complex-1, subunit 2"-was detected in the ad mutant. Although the genes responsible for Vg and Md were not definitively identified, we propose the candidate genes on the basis of their locations and phenotypes. (C) 2008 Elsevier Ltd. All rights reserved.