DIRECT SPECTROPHOTOMETRIC DETERMINATION OF INORGANIC SULFIDE IN BIOLOGICAL MATERIALS AND IN OTHER COMPLEX MIXTURES
DIRECT SPECTROPHOTOMETRIC DETERMINATION OF INORGANIC SULFIDE IN BIOLOGICAL MATERIALS AND IN OTHER COMPLEX MIXTURES
复制标题
DOI：
10.1016/0003-2697(71)90116-3
复制
发表时间：
1971-01-01
影响因子：
2.9
通讯作者：
GILBOAGARBER, N
中科院分区：
文献类型：
--
作者：
GILBOAGARBER, N
描述了一种改进的直接分光光度法，该方法适用于酶混合物和生物材料中硫化物的测定。该测定基于在酸性 N,N-二甲基对苯二胺和氯化铁存在下将硫化物特异性掺入亚甲蓝中。该方法的主要改进是通过硫化锌的定量沉淀和样品中可溶性成分的去除来实现的。该程序取代了在添加成色试剂之前用氮气夹带硫化氢的繁琐过程。这种非常简单的原位操作可有效防止样品介质中外来成分的干扰影响，并有助于进一步提高该方法的灵敏度。
An improved direct spectrophotometric method is described that is suited to determinations of sulfide in enzyme mixtures and in biological materials. The assay is based on the specific incorporation of sulfide into methylene blue in the presence of acidic, N,N-dimethyl-p-phenylenedi-amine and ferric chloride. The major improvement in the method is achieved by quantitative precipitation of zinc sulfide and removal of the soluble components of the sample. This procedure replaces the cumbersome entrainment of hydrogen sulfide with nitrogen gas prior to addition of the color-forming reagents. This very simple in situ manipulation is efficient in preventing the interfering effects of foreign constituents of the sample medium and contributes to a further increase in the sensitivity of the method.