Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain bicistronic s1 mRNA which encodes the minor capsid polypeptide sigma 1a and the nonstructural polypeptide sigma 1bNS.
Biosynthesis of reovirus-specified polypeptides. Molecular cDNA cloning and nucleotide sequence of the reovirus serotype 1 Lang strain bicistronic s1 mRNA which encodes the minor capsid polypeptide sigma 1a and the nonstructural polypeptide sigma 1bNS.
复制标题
呼肠孤病毒特异性多肽的生物合成。
DOI：
10.1016/0006-291x(86)90761-8
复制
发表时间：
1986
影响因子：
3.1
通讯作者：
Samuel,CE
中科院分区：
文献类型：
--
作者：
Munemitsu,SM;Atwater,JA;Samuel,CE
人呼肠孤病毒血清1型Lang株s1 mRNA编码次要衣壳细胞附着蛋白sigma 1a和非结构蛋白sigma 1bNS，用噬菌体M13在大肠杆菌中克隆为cDNA：mRNA异源双链体。Lang株s1基因全长1462个核苷酸，具有两个开放阅读框。第一个从nt 14开始，具有418个氨基酸的编码能力，足以解释sigma 1a;第二个从nt 75开始，具有119个氨基酸的编码能力，足以解释sigma 1bNS。将来源于s1 mRNA的cDNA克隆的Lang血清型s1序列与来源于S1 dsRNA基因组片段的cDNA克隆的Lang S1序列进行比较，明确确定呼肠孤病毒正链mRNA在结构上等同于dsRNA基因组片段的正链。
Human reovirus serotype 1 Lang strain s1 mRNA, which encodes the minor capsid cell attachment protein sigma 1a and the nonstructural protein sigma 1bNS, was cloned as a cDNA: mRNA heteroduplex in Eschericia coli using phage M13. The Lang strain s1 mRNA is 1462 nucleotides in length and possesses two open reading frames. The first begins at nt 14 and has a coding capacity of 418 amino acids, sufficient to account for sigma 1a; the second begins at nt 75 and has a coding capacity of 119 amino acids, sufficient to account for sigma 1bNS. Comparison of the Lang serotype s1 sequence derived from cDNA clones of s1 mRNA with the Lang S1 sequence derived from cDNA clones of the S1 dsRNA genome segment definitively establishes that reovirus plus-strand mRNA is structurally equivalent to the plus-strand of the dsRNA genome segment.