Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet‐cell differentiation from liver metastasis
Establishment and characterization of a human colonic mucinous carcinoma cell line with predominant goblet‐cell differentiation from liver metastasis
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DOI：
10.1111/j.1440-1827.2005.01868.x
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发表时间：
2005-09
影响因子：
2.2
通讯作者：
T. Yamachika;H. Nakanishi;K. Yasui;Y. Ikehara;T. Niwa;H. Wanibuchi;M. Tatematsu;S. Fukushima
中科院分区：
文献类型：
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作者：
T. Yamachika;H. Nakanishi;K. Yasui;Y. Ikehara;T. Niwa;H. Wanibuchi;M. Tatematsu;S. Fukushima
已经开发了许多人结肠直肠腺癌细胞系。然而，分化型结直肠癌细胞系，特别是杯状细胞分化型，是稀缺的。在本研究中，从一名日本女性的直肠粘液腺癌中建立了一种具有主要杯状细胞分化的新型结直肠腺癌细胞系（命名为COLM-6）。COLM-6细胞在培养物中以典型的上皮单层生长。它们在其表面表达表皮生长因子（EGF）受体和HER 2，因此，它们的生长受到EGF，转化生长因子（TGF）-α和heregulin的显著刺激。COLM-6细胞在裸鼠中形成具有典型粘液腺癌外观的肿瘤。这些皮下肿瘤的免疫组织化学分析表明，COLM-6细胞强烈表达MUC 2作为杯状细胞标志物，并在细胞核中表达Cdx 2。一些弱表达绒毛蛋白和碳酸酐酶1作为柱状吸收细胞标志物。它们的细胞质中腺瘤性结肠息肉病（APC）也呈阳性，并在其细胞质和细胞膜中表达β-连环蛋白，而无核积聚。这些结果表明COLM-6细胞系具有独特的生物学特性，可为研究结肠上皮细胞的生长分化机制以及结肠粘液腺癌的生物学行为提供有用的工具。
Many human colorectal adenocarcinoma cell lines have been developed. However, differentiated type colorectal cancer cell lines, particularly, the goblet‐cell differentiated type, are scarce. In the present study a novel colorectal adenocarcinoma cell line (designated as COLM‐6) with predominant goblet‐cell differentiation was established from the rectal mucinous adenocarcinoma of a Japanese woman. COLM‐6 cells grow in a typical epithelial monolayer in culture. They expressed epidermal growth factor (EGF) receptor and HER2 on their surface and accordingly, their growth was significantly stimulated by EGF, transforming growth factor (TGF)‐α and heregulin. COLM‐6 cells form tumor with typical mucinous adenocarcinomatous appearance in nude mice. Immunohistochemical analysis of these subcutaneous tumors demonstrated that COLM‐6 cells strongly express MUC2 as a goblet‐cell marker and Cdx2 in the nucleus. Some weakly express villin and carbonic anhydrase 1 as a columnar absorptive‐cell marker as well. They were also positive for adenomatous polyposis coli (APC) cytoplasmically and expressed β‐catenin in their cytoplasm and cell membrane without nuclear accumulation. These results indicate that COLM‐6 cell line has unique characteristics and may provide a useful tool to study the mechanism of growth and differentiation of colonic epithelium as well as the biological behavior of colorectal mucinous adenocarcinoma.