Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema
Significant correlation between endothelial nitric oxide synthase (eNOS) expression and alveolar repair in elastase-induced rat pulmonary emphysema
复制标题
DOI：
10.1007/s00595-012-0293-7
复制
发表时间：
2013-03-01
期刊：
影响因子：
2.5
通讯作者：
Sakakibara, Yuzuru
中科院分区：
文献类型：
--
作者：
Ito, Hiromichi;Matsushita, Shonosuke;Sakakibara, Yuzuru
血管生成因子，如内皮一氧化氮合酶（eNOS），被认为在肺气肿（PE）的修复中发挥重要作用;然而，相关因素的相关性尚未研究。本研究采用弹性蛋白酶诱导大鼠肺栓塞模型，分为A组（对照组）、B组（G-CSF组）、C组（肺栓塞组）和D组（肺栓塞+ G-CSF组）。在A、B、C和D组的比较中，肺泡密度分别为2.4 +/- A 0.2、2.4 +/- A 0.1、1.8 +/- A 0.1，2.5 +/-A0.1/100 μ m2（C与其他; p < 0.00001），小动脉数量分别为4.5 +/-A1.0、5.6 +/-A0.6、3.2 +/-A0.5、5.5 +/-A0.7/mm 2（C与其他; p < 0.05）。免疫组织化学染色（IHC）显示D组与C组中eNOS表达不同（p < 0.0001），蛋白质印迹显示D组与C组中eNOS、VEGF和FLT-1表达不同（p < 0.01，p < 0.05，p < 0.001），反映了血管生成对PE修复的贡献。内皮型一氧化氮合酶（eNOS）表达与肺泡密度和小动脉修复呈显著正相关，肺泡修复与弹性蛋白酶诱导的血管再生的eNOS表达呈正相关。
Angiogenic factors, such as endothelial nitric oxide synthase (eNOS), are thought to play an important role in the repair of pulmonary emphysema (PE); yet, the correlation of the factors involved has not been investigated. We conducted this study to clarify the positive correlation between eNOS expression and alveolar repair in PE recovery.We used elastase to induce PE in rats, which were divided into Groups A (Control), B (G-CSF), C (PE) and D (PE + G-CSF). G-CSF was injected for 12 days, 4 weeks after which the alveolar walls, arterioles, and angiogenic factors including eNOS were examined histopathologically and by western blotting.In comparing Groups A, B, C, and D, the alveolar density was 2.4 +/- A 0.2, 2.4 +/- A 0.1, 1.8 +/- A 0.1, 2.5 +/- A 0.1 per 100 mu m(2), respectively (C vs. others; p < 0.00001) and the number of arterioles was 4.5 +/- A 1.0, 5.6 +/- A 0.6, 3.2 +/- A 0.5, 5.5 +/- A 0.7/mm(2), respectively (C vs. others; p < 0.05). Immunohistochemical staining (IHC) revealed different eNOS expression in Group D versus Group C (p < 0.0001) and western blotting revealed different eNOS, VEGF, and FLT-1 expression in Group D versus Group C (p < 0.01, p < 0.05, p < 0.001), reflecting the contribution of angiogenesis to PE repair. eNOS showed a significantly positive correlation to alveolar density and arteriole repair.Alveolar repair was correlated positively with eNOS expression by vascular regeneration in elastase-induced rat PE.