Biosynthesis of highly enriched 13C-lycopene for human metabolic studies using repeated batch tomato cell culturing with 13C-glucose
Biosynthesis of highly enriched 13C-lycopene for human metabolic studies using repeated batch tomato cell culturing with 13C-glucose
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DOI：
10.1016/j.foodchem.2013.01.016
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发表时间：
2013-07-01
期刊：
影响因子：
8.8
通讯作者：
Erdman, John W., Jr.
中科院分区：
文献类型：
--
作者：
Moran, Nancy Engelmann;Rogers, Randy B.;Erdman, John W., Jr.
虽然在番茄（Solanum lycopersicum）产品及其消费者中发现了推定的防病番茄红素代谢物，但对哺乳动物的番茄红素代谢知之甚少。番茄细胞培养技术的发展为高浓度C-13番茄红素的生产提供了一种经济的工具，可用于人体生物利用度和代谢研究。为了提高hp-1番茄细胞系的C-13富集和标记番茄红素的产量，首先将培养物在C-13-葡萄糖培养基中连续培养三批，并产生增加比例的均匀标记番茄红素（14.3 +/-1.2%、39.6 +/-0.5%和48.9 +/- 1.5%），产率一致（5.8 - 9 mg/L）。基于葡萄糖利用率和番茄红素产量开发的优化的9天长的C-13加载和18天长的标记策略，产生具有93%C-13同位素纯度的C-13-番茄红素，并且55%的同位素异构体被均匀标记。此外，优化的丙酮和己烷提取导致从培养物中回收的番茄红素比标准提取增加四倍。(C)2013爱思唯尔有限公司保留所有权利。
While putative disease-preventing lycopene metabolites are found in both tomato (Solanum lycopersicum) products and in their consumers, mammalian lycopene metabolism is poorly understood. Advances in tomato cell culturing techniques offer an economical tool for generation of highly-enriched C-13-lycopene for human bioavailability and metabolism studies. To enhance the C-13-enrichment and yields of labelled lycopene from the hp-1 tomato cell line, cultures were first grown in C-13-glucose media for three serial batches and produced increasing proportions of uniformly labelled lycopene (14.3 +/- 1.2%, 39.6 +/- 0.5%, and 48.9 +/- 1.5%) with consistent yields (from 5.8 to 9 mg/L). An optimised 9-day-long C-13-loading and 18-day-long labelling strategy developed based on glucose utilisation and lycopene yields, yielded C-13-lycopene with 93% C-13 isotopic purity, and 55% of isotopomers were uniformly labelled. Furthermore, an optimised acetone and hexane extraction led to a fourfold increase in lycopene recovery from cultures compared to a standard extraction. (C) 2013 Elsevier Ltd. All rights reserved.