The clinical significance of EphA2 and Ephrin A-1 in epithelial ovarian carcinomas
The clinical significance of EphA2 and Ephrin A-1 in epithelial ovarian carcinomas
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DOI：
10.1016/j.ygyno.2005.06.036
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发表时间：
2005-11-01
影响因子：
4.7
通讯作者：
Suo, ZH
中科院分区：
文献类型：
--
作者：
Han, LQ;Dong, ZM;Suo, ZH
客观的。检测EPHA2和EphrinA-1蛋白和mRNA在上皮性卵巢癌/卵巢癌细胞系中的表达，探讨其对预后的价值。方法。为了验证免疫组织化学方法，使用 RTPCR、蛋白质印迹和免疫组织化学检查了两种卵巢癌细胞系（OVCAR3 和 SKOV3）的 EphA2 和 EphrinA-1 表达。然后评估 118 例晚期上皮性卵巢癌患者的肿瘤的 EPHA2 和 Ephrin A-1 蛋白表达，并将 30 例的冷冻组织用于激光捕获显微切割 (LCM) 辅助 RT-PCR RNA 分析。结果。 11 (9.3%)、67 (56.8%)、26 (22.0%) 和 14 (11.9%) 肿瘤分别表现出阴性、弱、中度和强 EpliA2 蛋白表达，而 3 (2.5%)、67 (56.8%)、32 (27.1%) 和 16 (13.8%) 肿瘤的 EpliA2 蛋白表达阴性、弱、中度和强。 Ephrin A-I 蛋白分别表达。通过LCM辅助RT-PCR方法分析的30个肿瘤中观察到不同量的mRNA表达。 EphA2 或 Ephrin A-1 表达水平较高与组织学分级较高之间存在相关趋势（两个因素的 P = 0.05）。 EphA2或Ephrin A-1的表达与年龄、组织学类型和FIGO分期之间没有观察到显着相关性。 EphA2蛋白表达水平较高的患者的生存期明显较短。 Cox多变量分析显示术后残留肿瘤、组织学类型和EphA2蛋白表达具有独立的预后意义。结论。高水平的 EphA2 蛋白表达与较短的患者生存期显着相关，EphA2 受体是卵巢癌有价值的预后标志物。 (c) 2005 Elsevier Inc. 保留所有权利。
Objective. To examine the expressions of the protein and mRNA of EPHA2 and EphrinA-1 in epithelial ovarian carcinomas/ovarian cancer cell lines and explore their prognostic value.Methods. To validate the immunohistochemical method, two ovarian cancer cell lines (OVCAR3 and SKOV3) were examined with RTPCR, Western blot, and immunohistochemistry for EphA2 and EphrinA-1 expressions. Tumors from 118 patients with advanced epithelial ovarian cancer were then evaluated for EPHA2 and Ephrin A-1 protein expression, and frozen tissues from 30 cases Were used for laser capture microdissection (LCM) assistant RT-PCR RNA analysis.Results. 11 (9.3%), 67 (56.8%), 26 (22.0%), and 14 (11.9%) tumors demonstrated negative, weak, moderate, and strong EpliA2 protein expressions, respectively, while 3 (2.5%), 67 (56.8%) 32 (27.1%), and 16 (13.8%) tumors were negative, weak, moderate, and strong for Ephrin A-I protein expression, respectively. Variable amount of mRNA expression was observed in the 30 tumors analyzed by the method of LCM assistant RT-PCR. There was a trend for association between higher levels of either EphA2 or Ephrin A-1 expression and higher histological grade (P = 0.05 for both factors). No significant correlation between the expressions of EphA2 or Ephrin A-1 and age, histological type, and FIGO stage was observed. Patients with higher levels of EphA2 protein expressions had significantly shorter survival. Cox multivariate analyses revealed that residual tumor after surgery, histological type, and EphA2 protein expression were of independent prognostic significance.Conclusions. High level of EphA2 protein expression is significantly associated with a shorter patient survival and EphA2 receptor is a valuable prognostic marker for ovarian carcinoma. (c) 2005 Elsevier Inc. All rights reserved.