Thioacetamide- and carbon tetrachloride-induced liver cirrhosis.
Thioacetamide- and carbon tetrachloride-induced liver cirrhosis.
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硫代乙酰胺和四氯化碳诱发的肝硬化。
DOI：
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发表时间：
1989
期刊：
影响因子：
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通讯作者：
Stig Bengmark
中科院分区：
文献类型：
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作者：
H. Dashti;Bengt Jeppsson;I. Hagerstrand;Björn Hultberg;U. Srinivas;M. Abdulla;Stig Bengmark
本文研究了两种诱导大鼠肝硬变的方法。根据Proctor和Chatamra的标准，灌胃给予CCl4 16周，并与同期在饮用水中给予硫代乙酰胺(0.3g/L)进行比较。CCl_4诱导6/8动物出现小结节性肝硬变，死亡率为27%。硫代乙酰胺诱导的8只动物中有6只发生了肝硬变，无死亡。组织学图像略有不同，CCl4组有更多的坏死和细胞肿胀，而硫代乙酰胺组有更多的核异型性和增殖。生化方面，两组患者的血浆天冬氨酸氨基转移酶水平均升高。溶酶体酶β-氨基己糖苷酶(β-NAG)在硫代乙酰胺动物体内表现为一过性升高，而β-葡萄糖醛酸苷酶则降低。CCl_4诱导的肝硬变导致β-NAG升高。两组大鼠血浆锌含量均下降，CCl4组大鼠肝脏锌含量下降，而硫代乙酰胺组大鼠肝脏锌含量持续升高。结论：口服硫代乙酰胺是一种简便可靠的实验性肝硬变模型。组织学形态和某些生化指标的差异可能是由于硫代乙酰胺和CCl_4作用机制不同所致。
Two methods of inducing liver cirrhosis in the rat were studied. Intragastric administration of CCl4 for 16 weeks according to Proctor and Chatamra was compared to the administration of thioacetamide in the drinking water (0.3 g/l) for the same period. CCl4 administration induced micronodular cirrhosis in 6/8 animals with a 27% mortality. Thioacetamide induced cirrhosis in 6/8 animals without mortality. The histologic pictures differed somewhat in that the CCl4 group exhibited more necrosis and cellular swelling while the thioacetamide group had more nuclear atypias and proliferation. Biochemically both groups had elevated plasma levels of aspartate aminotransferase. The lysosomal enzyme beta-hexosaminidase (beta-NAG) showed a transient increase in the thioacetamide animals, while beta-glucuronidase decreased. CCl4-induced cirrhosis led to an increase in beta-NAG. Plasma zinc decreased in both groups as well as liver zinc content in the CCl4 group, while there was a continuous elevation of liver zinc in the thioacetamide group. We conclude that oral administration of thioacetamide is a simple and reliable method of inducing experimental liver cirrhosis. The differences in histological appearances and some biochemical parameters may be caused by the different mechanisms of action of thioacetamide and CCl4.