Comparison of the biological effects of (18)F at different intracellular levels.
Comparison of the biological effects of (18)F at different intracellular levels.
复制标题
(18)F在不同细胞内水平的生物学效应比较。
DOI：
10.1016/j.bbrc.2014.09.136
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发表时间：
2014
期刊：
影响因子：
--
通讯作者：
Mori H.
中科院分区：
文献类型：
--
作者：
Kashino G;Hayashi K;Douhara K;Kobashigawa S;Mori H.
我们在此研究了用18f标记的药物处理细胞的生物效应，用于正电子发射断层扫描（PET）。细胞内18f分布与受损DNA水平之间的关系尚未得到详细阐明。用氟脱氧葡萄糖（FDG）和氟离子（HF）两种18f标记药物处理培养细胞（中国仓鼠卵巢细胞）。FDG能有效地在细胞内积累，而HF则不能。为了研究DNA双链断裂（DSB）的诱导作用，我们测量了53BP1在DNA双链断裂位点形成的病灶数量。结果显示，虽然放射性水平相同，但18f - fdg处理的细胞对53BP1灶的诱导强于18f - hf处理的细胞。18f - fdg组细胞的克隆成活率明显低于18f - hf组。我们的结论是，18f在细胞中的有效积累导致了更强的生物效应，因为通过诱导DNA DSB更严重的细胞致命性。
We herein examined the biological effects of cells treated with18F labeled drugs for positron emission tomography (PET). The relationship between the intracellular distribution of18F and levels of damaged DNA has yet to be clarified in detail. We used culture cells (Chinese Hamster Ovary cells) treated with two types of18F labeled drugs, fluorodeoxyglucose (FDG) and fluorine ion (HF). FDG efficiently accumulated in cells, whereas HF did not. To examine the induction of DNA double strand breaks (DSB), we measured the number of foci for 53BP1 that formed at the site of DNA DSB. The results revealed that although radioactivity levels were the same, the induction of 53BP1 foci was stronger in cells treated with18F-FDG than in those treated with18F-HF. The clonogenic survival of cells was significantly lower with18F-FDG than with18F-HF. We concluded that the efficient accumulation of18F in cells led to stronger biological effects due to more severe cellular lethality via the induction of DNA DSB.