Genome-wide identification, classification, evolutionary expansion and expression analyses of homeobox genes in rice
Genome-wide identification, classification, evolutionary expansion and expression analyses of homeobox genes in rice
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DOI：
10.1111/j.1742-4658.2008.06424.x
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发表时间：
2008-06-01
期刊：
影响因子：
5.4
通讯作者：
Khurana, Jitendra P.
中科院分区：
文献类型：
--
作者：
Jain, Mukesh;Tyagi, Akhilesh K.;Khurana, Jitendra P.
同源框基因在植物生长发育的各个方面起着重要的调节作用。在本研究中，我们在水稻基因组中鉴定了107个同源异型盒基因，并根据它们的结构域组成和系统发育分析将它们划分为10个不同的亚家族。大量的同源异型盒基因位于水稻基因组的重复片段中，这表明同源异型盒基因家族的扩大在很大程度上可能是由于水稻基因组中的片段重复所致。此外，通过基因芯片分析这些基因在不同组织以及营养和生殖发育的不同阶段的表达谱。确定了几个在穗和种子发育的不同阶段有优势表达的基因。研究发现，在不同的非生物胁迫条件下，至少有37个同源异型盒基因显著差异表达(超过两倍；P<；0.05)。研究结果表明，同源框基因在水稻生殖发育和非生物胁迫信号传递中起着关键作用，并将有助于筛选具有农艺重要性的候选基因进行功能验证。
Homeobox genes play a critical role in regulating various aspects of plant growth and development. In the present study, we identified a total of 107 homeobox genes in the rice genome and grouped them into ten distinct subfamilies based upon their domain composition and phylogenetic analysis. A significantly large number of homeobox genes are located in the duplicated segments of the rice genome, which suggests that the expansion of homeobox gene family, in large part, might have occurred due to segmental duplications in rice. Furthermore, microarray analysis was performed to elucidate the expression profiles of these genes in different tissues and during various stages of vegetative and reproductive development. Several genes with predominant expression during various stages of panicle and seed development were identified. At least 37 homeobox genes were found to be differentially expressed significantly (more than two-fold; P < 0.05) under various abiotic stress conditions. The results of the study suggest a critical role of homeobox genes in reproductive development and abiotic stress signaling in rice, and will facilitate the selection of candidate genes of agronomic importance for functional validation.