pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor
pH level has a strong impact on population dynamics of the yeast Yarrowia lipolytica and oil micro-droplets in multiphasic bioreactor
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DOI：
10.1093/femsle/fny173
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发表时间：
2018-08-01
影响因子：
2.1
通讯作者：
Delvigne, Frank
中科院分区：
文献类型：
--
作者：
Bouchedja, Doria Naila;Danthine, Sabine;Delvigne, Frank
产油酵母解脂耶氏酵母具有利用油脂作为碳源的能力，使其成为设计基于可再生底物的替代生物工艺的有前途的细胞工厂。然而，这种多相生物反应器的设计相当复杂，并且在考虑疏水基质上的油-水混合物的乳化、发泡和细胞生长/生理时存在一些限制。本研究旨在阐明pH变化对培养基理化性质和细胞生理的影响。确实观察到，pH值为6时，细胞生长速度和细胞内脂质积累最优。此外，泡沫明显减少。为了避免生物反应器中过度起泡，在不损害细胞生理的前提下，采用仅作用于培养基物理结构的替代工艺，似乎是一种有效的替代常规化学消泡剂的方法。
The oleaginous yeast Yarrowia lipolytica has the ability to use oils and fats as carbon source, making it a promising cell factory for the design of alternative bioprocesses based on renewable substrates. However, such a multiphasic bioreactor design is rather complex and leads to several constraints when considering emulsification of the oil-in-water mixture, foaming and cell growth/physiology on hydrophobic substrate. This study aims to shed light on the effect of pH changes on the physico-chemical properties of the cultivation medium and on cell physiology. It was indeed observed that at a pH value of 6, cell growth rate and intracellular lipid accumulation were optimized. Additionally, foaming was significantly reduced. In order to avoid over foaming in bioreactor, without impairing cell physiology, the use of alternative processes that can only act on the physical structure of culture medium, seems to be an effective alternative to usual chemical anti-foam agents.