Atlas of olfactory organs of Drosophila melanogaster 2.: Internal organization and cellular architecture of olfactory sensilla
Atlas of olfactory organs of Drosophila melanogaster 2.: Internal organization and cellular architecture of olfactory sensilla
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DOI：
10.1016/s1467-8039(00)00028-1
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发表时间：
2000-01-01
影响因子：
2
通讯作者：
Steinbrecht, RA
中科院分区：
文献类型：
--
作者：
Shanbhag, SR;Müller, B;Steinbrecht, RA
用电子显微镜对黑腹果蝇的触角和上颌触须进行了连续切片冷冻观察。表皮细胞的数量大致等于感受器的数量，但感受器稀少或缺失的区域除外。每个表皮细胞形成约两个无神经支配的小刺，一个突出的表皮下间隙和一个明显的基底迷宫，表明液体通过感觉上皮的运输速率很高。毛状感受器、中间感受器和基本感受器的内部组织和精细结构非常相似。受体细胞体由延伸至内部树突节段的薄胶质鞘包裹。再往远端，氧原细胞在树突周围形成一个套筒，但没有细胞外树突鞘。在角质层器官的底部，睫状体周围的内感觉器-淋巴空间和外树突节周围的外感觉器-淋巴空间与由毛原细胞和原细胞形成的大的外感觉器-淋巴空间汇合。所有三种辅助细胞都具有分泌和运输细胞的许多特征，但仅向血淋巴窦延伸薄的基突。耳蜗感觉器的平面和精细结构在以下几个方面有所不同：(1)树突纤毛节位于角质层器官基部以下较其他类型的感觉器深；(2)总有突出的树突鞘，将内外感觉淋巴间隙完全分开；(3)辅助细胞的顶端微片更精细，但几乎没有自由的感受器淋巴间隙；(4)总有四个而不是三个辅助细胞。树突内、外节的直径，受体细胞的大小，以及受体和辅助细胞核的大小，都有形态测量数据。从感受器功能和刺激转导相关蛋白的定位等方面论述了果蝇嗅感受器特殊的精细结构特征。2001爱思唯尔科学有限公司版权所有。
Antennae and maxillary palps of Drosophila melanogaster were studied with the electron microscope on serial sections of cryofixed specimens. The number of epidermal cells roughly equals the number of sensilla, except for regions where the latter are scarce or absent. Each epidermal cell forms about two non-innervated spinules, a prominent subcuticular space and a conspicuous basal labyrinth, suggesting a high rate of fluid transport through the sensory epithelium. The internal organization and fine structure of trichoid, intermediate and basiconic sensilla is very similar. Receptor cell somata are invested by thin glial sheaths extending distad to the inner dendritic segments. Further distally, the the cogen cell forms a sleeve around the dendrites, but an extracellular dendrite sheath is absent. At the base of the cuticular apparatus, the inner sensillum-lymph space around the ciliary and outer dendritic segments is confluent with the large outer sensillum-lymph space formed by the trichogen and tormogen cells. All three auxiliary cells exhibit many features of secretory and transport cells but extend only thin basal processes towards the haemolymph sinus. The bauplan and fine structure of coeloconic sensilla differs in the following aspects: (1) the ciliary segment of the dendrites is located deeper below the base of the cuticular apparatus than in the other sensillum types; (2) a prominent dendrite sheath is always present, separating inner and outer sensillum-lymph spaces completely; (3) the apical microlamellae of the auxiliary cells are more elaborate, but free sensillum-lymph spaces are almost absent; (4) there are always four not three auxiliary cells. Morphometric data are presented on the diameter of inner and outer dendritic segments and on the size of receptor cells, as well as of the receptor and auxiliary cell nuclei. The special fine structural features of Drosophila olfactory sensilla are discussed under the aspects of sensillar function and the localization of proteins relevant for stimulus transduction. (C) 2001 Elsevier Science Ltd. All rights reserved.