HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR - CHARACTERIZATION OF RAT AND MOUSE CDNA CLONES, PROTEIN DOMAIN CONSERVATION ACROSS SPECIES, AND TRANSCRIPT EXPRESSION IN TISSUES
HEPARIN-BINDING EGF-LIKE GROWTH-FACTOR - CHARACTERIZATION OF RAT AND MOUSE CDNA CLONES, PROTEIN DOMAIN CONSERVATION ACROSS SPECIES, AND TRANSCRIPT EXPRESSION IN TISSUES
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DOI：
10.1006/bbrc.1993.1020
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发表时间：
1993-01-15
影响因子：
3.1
通讯作者：
EZEKOWITZ, RAB
中科院分区：
文献类型：
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作者：
ABRAHAM, JA;DAMM, D;EZEKOWITZ, RAB
获得了编码大鼠和小鼠形式的肝素结合EGF样生长因子（HB-EGF）的克隆，HB-EGF是平滑肌细胞、成纤维细胞和角质形成细胞的有效有丝分裂原，其被认为源自跨膜前体。在从这些cDNA预测的HB-EGF前体序列中，发现与人或猴HB-EGF（73 - 76%序列同一性）相比，对应于分泌（“成熟”）因子的区域代表最不保守的区域之一。高序列保守性区域包括建议的跨膜和跨膜结构域，以及建议的肝素结合区域内的“成熟”因子。使用HB-EGF克隆作为探针的北方印迹实验揭示了HB-EGF转录物在多种组织中的表达，特别是肺、骨骼肌、脑和心脏。
Clones were obtained that encode the rat and mouse forms of heparin-binding EGF-like growth factor (HB-EGF), a potent mitogen for smooth muscle cells, fibroblasts and keratinocytes that is proposed to be derived from a transmembrane precursor. Within the HB-EGF precursor sequences predicted from these cDNAs, the region corresponding to the secreted ("mature") factor was found to represent one of the least well conserved areas when compared to human or monkey HB-EGF (73 - 76% sequence identity). Regions of high sequence conservation included the proposed juxtamembrane and transmembrane domains, as well as a proposed heparin-binding region within the "mature" factor. Northern blotting experiments using the HB-EGF clones as probes revealed HB-EGF transcript expression in multiple tissues, particularly lung, skeletal muscle, brain, and heart.