Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis
Development of nineteen novel polymorphic microsatellite loci of Clupeichthys aesarnensis
复制标题
伊萨十九个新多态性微卫星位点的开发
DOI：
10.1007/s12686-013-9887-7
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发表时间：
2013
影响因子：
1.1
通讯作者：
A.(他5名)
中科院分区：
文献类型：
--
作者：
Koizumi;N.;Morioka;S.;Mori;A.(他5名)
研究人员从中华月牙鱼（clupeichthys aesarnensis）基因组中提取并鉴定了19个新的多态微卫星位点。利用侧翼PCR引物对30个个体进行分析，发现所有位点均具有多态性，每个位点的等位基因数在2 ~ 15个之间。观察杂合度在0.033 ~ 0.933之间，期望杂合度在0.033 ~ 0.898之间。所有位点均符合Hardy-Weinberg平衡，所有位点组合均不存在明显的连锁不平衡，没有位点存在空等位基因。这19个微卫星基因座可为今后对牛茅属群体遗传分化和结构的研究提供参考。aesarnensis。
We developed and characterized a total of 19 novel polymorphic microsatellite loci from the genome ofClupeichthys aesarnensis. Flanking PCR primers were developed and used to show that all loci were polymorphic with the number of observed alleles per locus in 30 individuals ranging from two to 15. The observed heterozygosity ranged from 0.033 to 0.933, while the expected heterozygosity varied from 0.033 to 0.898. All loci conformed to the Hardy–Weinberg equilibrium, none of the loci combinations showed significant linkage disequilibrium, and no loci showed evidence of null alleles. These 19 microsatellite loci can be useful for conducting future investigations into the genetic differentiation and structure of populations ofC. aesarnensis.