Characterisation of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR
Characterisation of diarrhoeagenic Escherichia coli clones by ribotyping and ERIC-PCR
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DOI：
10.1099/00222615-47-3-227
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发表时间：
1998-03-01
影响因子：
3
通讯作者：
Trabulsi, LR
中科院分区：
文献类型：
--
作者：
Dalla-Costa, LM;Irino, K;Trabulsi, LR
核糖体分型和肠杆菌重复基因间浓度-聚合酶链反应（ERIC-PCR）的能力，区分属于26个不同血清型的122株产酶大肠杆菌克隆进行了评价。26个血清型对应24个核糖体型和25个ERIC型。多位点酶电泳、ERIC-PCR和核糖体分型的相关性为c。90%为优势核糖型。O 55：H7和O 157：H7等相关克隆具有相似的核糖体类型，并在树状图中聚在一起，而肠致病性E.大肠杆菌（EPEC）和肠出血性大肠杆菌（E.大肠杆菌（EHEC）被包括在不同的分支。结果表明，应用这两种简单的技术作为工具，以确定克隆的Escherichia coli的可能性。杆菌
The ability of ribotyping and enterobacterial repetitive intergenic concensus-polymerase chain reaction (ERIC-PCR) to discriminate diarrhoeagenic Escherichia coli clones of 122 strains belonging to 26 distinct serotypes was evaluated. The 26 serotypes corresponded to 24 ribotypes and 25 ERIC-types. Correlation between multilocus enzyme electrophoresis, ERIC-PCR and ribotyping was c. 90% for the dominant ribotypes. Related clones such as O55:H7 and O157:H7 presented similar ribotypes and clustered together in a dendrogram, and the two divergent clonal groups of enteropathogenic E. coli (EPEC) and enterohaemorrhagic E. coli (EHEC) were included in distinct branches. The results suggest the possibility of applying these two simpler techniques as tools to identify clones of diarrhoeagenic E. coli.