Early-warning electrochemical biosensor system for environmental monitoring based on enzyme inhibition

Early-warning electrochemical biosensor system for environmental monitoring based on enzyme inhibition
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DOI:
10.1016/j.snb.2004.02.039
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发表时间:
2005-02-14
影响因子:
8.4
通讯作者:
Jaffrezic-Renault, N
Jaffrezic-Renault, N
中科院分区:
化学1区
文献类型:
--
作者:
Dzyadevych, SV;Soldatkin, AP;Jaffrezic-Renault, N

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已经开发出基于固定化胆碱酯酶的电位和电导生物传感器。通过酶抑制机制实现了一些有机磷农药(甲基对硫磷、甲基对氧磷、乙基对氧磷、敌百虫、二异丙基氟磷酸酯)和氨基甲酸酯类农药(克百威)的检测。开发的生物传感器系统与传统分析技术(高效液相色谱和基于Lumistox测试的灵敏度比较) 费氏弧菌发光细菌)已表明生物传感器测量程序非常适合快速环境控制。讨论了所创建系统的分析特征、其优点和缺点。 (C) 2004 Elsevier B.V. 保留所有权利。
Potentiometric and conductometric biosensors based on immobilised cholinesterases have been developed. Detection of some organophosphorous pesticides (methyl parathion, methyl paraoxon, ethyl paraoxon, trichlorphon, diisopropyl fluorophosphates) and carbamate pesticide (carbofuran) have been realised through enzyme inhibition mechanisms.Comparison of sensitivity of the biosensor systems developed with that of traditional analytical techniques (high performance liquid chromatography and Lumistox test based on Vibrio fischeri luminescent bacteria) has shown that biosensor measuring procedures are well suited for fast environmental control. Analytical characteristics of the systems created, their advantages and shortcomings are discussed. (C) 2004 Elsevier B.V. All rights reserved.