Temporal and spatial expression patterns for the tumor suppressor Maspin and its binding partner interferon regulatory factor 6 during breast development.
Temporal and spatial expression patterns for the tumor suppressor Maspin and its binding partner interferon regulatory factor 6 during breast development.
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DOI：
10.1111/j.1440-169x.2009.01110.x
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发表时间：
2009-06
期刊：
影响因子：
--
通讯作者：
Hendrix MJ
中科院分区：
文献类型：
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作者：
Bailey CM;Margaryan NV;Abbott DE;Schutte BC;Yang B;Khalkhali-Ellis Z;Hendrix MJ
干扰素调节因子6（IRF 6）是转录因子的干扰素调节因子家族的非典型成员。我们最近确定IRF 6作为一种新的Maspin相互作用的蛋白质在乳腺上皮细胞。Maspin是乳腺中的肿瘤抑制因子，并且也与乳腺形态发生有关。为了探索IRF 6与Maspin在乳腺生长和分化过程中的可能作用，我们使用体外和体内方法的组合检查了IRF 6和Maspin在子宫后乳腺发育过程中的表达。数据显示，IRF 6和Maspin的表达在整个乳腺发育过程中受到时间和空间的调节，这两种蛋白质的最大表达发生在完全分化的泌乳小叶肺泡细胞中。我们进一步表明，IRF 6采用内腔定位模式后，完整的上皮细胞极化和IRF 6分泌到牛奶中提出了新的证据。这些结果支持IRF 6和Maspin对乳腺上皮细胞分化很重要的假设，并推进了我们对正常乳腺发育过程中Maspin-IRF 6伙伴关系的理解。
Interferon regulatory factor 6 (IRF6) is a non-canonical member of the interferon regulatory factor family of transcription factors. We recently identified IRF6 as a novel Maspin-interacting protein in mammary epithelial cells. Maspin is a tumor suppressor in the breast and has also been implicated in mammary gland morphogenesis. To explore a possible role for IRF6 in conjunction with Maspin during mammary gland growth and differentiation, we examined the expression of IRF6 and Maspin during post-utero mammary gland development using a combination of in vitro and in vivo approaches. The data revealed that the expression of IRF6 and Maspin is temporally and spatially regulated throughout mammary gland development, with maximal expression of both proteins occurring in fully differentiated, lactating lobuloalveolar cells. We further show that IRF6 adopts a lumenal localization pattern following complete epithelial cell polarization and present new evidence for the secretion of IRF6 into the milk. These results support the hypothesis that IRF6 and Maspin are important for mammary epithelial cell differentiation, and advance our understanding of the Maspin-IRF6 partnership during normal mammary gland development.