ANAEROBIC GROWTH OF GONOCOCCI DOES NOT ALTER THEIR OPA-MEDIATED INTERACTIONS WITH HUMAN NEUTROPHILS
ANAEROBIC GROWTH OF GONOCOCCI DOES NOT ALTER THEIR OPA-MEDIATED INTERACTIONS WITH HUMAN NEUTROPHILS
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DOI：
10.1128/iai.60.5.1793-1799.1992
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发表时间：
1992-05-01
影响因子：
3.1
通讯作者：
REST, RF
中科院分区：
文献类型：
--
作者：
FRANGIPANE, JV;REST, RF
在亚硝酸盐存在下厌氧生长的淋球菌诱导至少三种新的外膜蛋白(PAN 1至3)的表达。虽然淋病过程中淋球菌表达PAN-1，但PAN蛋白的功能仍不清楚。在没有血清的情况下，具有不透明相关(OpA，以前的PII)外膜蛋白的淋球菌附着、刺激并被人类中性粒细胞吞噬杀死。缺乏OpA蛋白的淋球菌没有表现出这些活性。我们研究了PAN蛋白或厌氧淋球菌的任何其他特征是否改变了非纤毛、Opa+或Opa-淋球菌附着、刺激中性粒细胞或被中性粒细胞吞噬杀死的能力。菌株F62在十二烷基硫酸钠-聚丙烯酰胺凝胶上的相对迁移率确定的Opa4的表达似乎没有被厌氧生长改变，正如以前看到的(V.L.Clark，L.A.Campbell，D.A.Palermo，T.M.Evans，和K.W.Klimpel，感染免疫。55：1359-1364,1987)。厌氧和需氧的Opa+淋球菌对中性粒细胞的黏附和刺激程度相同。同样，厌氧性和需氧性奥帕-淋球菌对中性粒细胞的粘附性和刺激性也同样差。最后，厌氧和需氧的Opa+淋球菌对中性粒细胞的吞噬杀伤同样敏感，而厌氧和需氧的Opa+淋球菌对杀伤同样敏感。因此，OpA蛋白在介导淋球菌与人中性粒细胞相互作用中的作用似乎不会因厌氧生长而改变，PAN蛋白或厌氧淋球菌的其他隐蔽特性似乎不调节或调节这些现象。
Gonococci grown anaerobically (anaerobic gonococci) in the presence of nitrite induce the expression of at least three novel outer membrane proteins (PANs 1 to 3). Although PAN 1 is expressed by gonococci during gonorrhea, the function of the PAN proteins remains unknown. In the absence of serum, gonococci possessing opacity-associated (Opa, formerly PII) outer membrane proteins adhere to, stimulate, and are phagocytically killed by human neutrophils. Gonococci lacking Opa proteins demonstrate none of these activities. We investigated whether the PAN proteins, or any other characteristics of anaerobic gonococci, altered the ability of nonpiliated, Opa+ or Opa- gonococci to adhere to, stimulate, or be phagocytically killed by neutrophils. The expression of Opa4 by strain F62, as determined by its relative mobility on sodium dodecyl sulfate-polyacrylamide gels, appeared to be unaltered by anaerobic growth, as seen previously (V. L. Clark, L. A. Campbell, D. A. Palermo, T. M. Evans, and K. W. Klimpel, Infect Immun. 55: 1359-1364, 1987). Anaerobic and aerobic Opa+ gonococci adhered to and stimulated neutrophils to the same extent. Similarly, anaerobic and aerobic Opa- gonococci adhered to and stimulated neutrophils equally poorly. Finally, anaerobic and aerobic Opa+ gonococci were equally sensitive to phagocytic killing by neutrophils, while anaerobic and aerobic Opa- gonococci were equally resistant to killing. Thus, the role of Opa proteins in mediating the interactions of gonococci with human neutrophils appears unaltered by anaerobic growth, and the PAN proteins, or other cryptic properties of anaerobic gonococci, do not seem to modulate or mediate these phenomena.