Focal Cerebral Ischemia in Rats: Effect of Hemodilution with α-α Cross-Linked Hemoglobin on CBF
Focal Cerebral Ischemia in Rats: Effect of Hemodilution with α-α Cross-Linked Hemoglobin on CBF
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大鼠局灶性脑缺血：α-α 交联血红蛋白血液稀释对 CBF 的影响
DOI：
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
K. Bradley
中科院分区：
文献类型：
--
作者：
D. J. Cole;R. Schell;R. Przybelski;J. Drummond;K. Bradley
血液稀释作为脑缺血治疗的成功有限。当使用非氧结合液体时，血液稀释引起的CBF增加的治疗效果被伴随的氧含量减少所抵消。评估了用地司匹林 α-α 交联血红蛋白 (DCLHb) 进行血液稀释对大脑中动脉闭塞期间 CBF 的影响。将大鼠血液稀释至以下血细胞比容（Hct）之一：（a）44/Hct，（b）37/Hct，（c）30/Hct，（d）23/Hct，（e）16/Hct，或（f）9/Hct。缺血10分钟后，用14C-碘安替比林测定CBF。评估冠状脑切片的 CBF 为 0-10 和 11-20 ml 100 g−1 min−1 的区域。此外，还计算了氧气输送量。在缺血区的中心，与44/Hct和37/Hct组相比，30/Hct、23/Hct和16/Hct组的两个低CBF区域均较少；与其他五组相比，9/Hct 组的两个面积均较小（p < 0.05）。对于闭塞对侧半球，血细胞比容和氧输送之间存在直接相关性。然而，对于闭塞同侧半球，氧输送随着血细胞比容降低而增加（44/Hct，8.6 ± 0.3 vs. 9/Hct，13.6 ± 0.4 [平均值±SD，ml 100 g−1 min−1]）。这项研究的结果支持这样的假设：用 DCLHb 进行血液稀释可降低局灶性脑缺血的程度。
Hemodilution has had limited success as a treatment of cerebral ischemia. When using a nonoxygen binding fluid, the therapeutic efficacy of hemodilution-induced increases in CBF are offset by concomitant decreases in oxygen content. The effect of hemodilution, with diaspirin α-α cross-linked hemoglobin (DCLHb), on CBF during middle cerebral artery occlusion was assessed. Rats were hemodiluted to one of the following hematocrits (Hct): (a) 44/Hct, (b) 37/Hct, (c) 30/Hct, (d) 23/Hct, (e) 16/Hct, or (f) 9/Hct. After 10 min of ischemia, CBF was determined with 14C-iodoantipyrine. Coronal brain sections were evaluated for areas with a CBF of 0–10 and 11–20 ml 100 g−1 min−1. In addition, oxygen delivery was calculated. In the center of the ischemic zone, both areas of low CBF were less in the 30/Hct, 23/Hct, and 16/Hct groups compared with the 44/Hct and 37/Hct groups; and both areas were less in the 9/Hct group compared with the other five groups (p < 0.05). For the hemisphere contralateral to occlusion, there was a direct correlation between hematocrit and oxygen delivery. However, for the hemisphere ipsilateral to occlusion, oxygen delivery increased as hematocrit decreased (44/Hct, 8.6 ± 0.3 vs. 9/Hct, 13.6 ± 0.4 [mean ± SD, ml 100 g−1 min−1]). The results of this study support a hypothesis that hemodilution with DCLHb decreases the extent of focal cerebral ischemia.
DOI：
10.1016/s0021-9258(18)67605-7
发表时间：
1986-07
期刊：
The Journal of biological chemistry
影响因子：
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作者：
R. Chatterjee;E. Welty;R. Walder;S. Pruitt;P. Rogers;A. Arnone;J. A. Walder
通讯作者：
R. Chatterjee;E. Welty;R. Walder;S. Pruitt;P. Rogers;A. Arnone;J. A. Walder
放射自显影血流示踪剂的死后扩散。
影响因子：
2.9
作者：
Greenberg,JH;LoBrutto,C;Lombard,KM;Chen,J
通讯作者：
Chen,J
等容血液稀释在实验性局灶性脑缺血中的应用。
影响因子：
4.1
作者：
Tu,YK;Heros,RC;Karacostas,D;Liszczak,T;Hyodo,A;Candia,G;Zervas,NT;Lagree,K
通讯作者：
Lagree,K