Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents
Ginseng extracts modulate mitochondrial bioenergetics of live cardiomyoblasts: a functional comparison of different extraction solvents
复制标题
DOI：
10.1016/j.jgr.2018.02.002
复制
发表时间：
2019-10-01
影响因子：
6.3
通讯作者：
Tsim, Karl Wah Keung
中科院分区：
文献类型：
--
作者：
Huang, Yun;Kwan, Kenneth Kin Leung;Tsim, Karl Wah Keung
背景资料：人参是五加科植物人参的根，在亚洲被用作草药和功能性食品已有数千年的历史。根据传统中医，人参是最广泛使用的“补气”草药，通过抗氧化应激，影响能量代谢和改善线粒体功能来提供滋补和预防作用。很少有报道系统地测量了人参处理后细胞线粒体的生物能量学。方法：在这里，H9C2细胞系，一种大鼠心肌母细胞，用人参提取物处理，人参提取物用不同极性的溶剂提取，即，结果：人参90%乙醇提取物对H9C2心肌细胞线粒体呼吸具有最强的抗氧化和促进作用，对H9C2心肌细胞的保护作用最强。通过增加应激H9C2细胞的备用呼吸能力高达健康细胞的三倍，人参的90%乙醇提取物大大提高了心肌细胞对氧化损伤的耐受性。结果表明，人参的低极性提取物是最好的提取物，在线粒体呼吸过程中调节培养心肌细胞的耗氧率。(C)2018韩国人参协会，由Elsevier Korea LLC出版。
Background: The root of Panax ginseng, a member of Araliaceae family, has been used as herbal medicine and functional food in Asia for thousands of years. According to Traditional Chinese medicine, ginseng is the most widely used "Qi-invigorating" herbs, which provides tonic and preventive effects by resisting oxidative stress, influencing energy metabolism, and improving mitochondrial function. Very few reports have systematically measured cell mitochondrial bioenergetics after ginseng treatment.Methods: Here, H9C2 cell line, a rat cardiomyoblast, was treated with ginseng extracts having extracted using solvents of different polarity, i.e., water, 50% ethanol, and 90% ethanol, and subsequently, the oxygen consumption rate in healthy and tert-butyl hydroperoxideetreated live cultures was determined by Seahorse extracellular flux analyzer.Results: The 90% ethanol extracts of ginseng possessed the strongest antioxidative and tonic activities to mitochondrial respiration and therefore provided the best protective effects to H9C2 cardiomyocytes. By increasing the spare respiratory capacity of stressed H9C2 cells up to three-folds of that of healthy cells, the 90% ethanol extracts of ginseng greatly improved the tolerance of myocardial cells to oxidative damage.Conclusion: These results demonstrated that the low polarity extracts of ginseng could be the best extract, as compared with others, in regulating the oxygen consumption rate of cultured cardiomyocytes during mitochondrial respiration. (C) 2018 The Korean Society of Ginseng, Published by Elsevier Korea LLC.