Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications.
Transcriptional regulation with CRISPR-Cas9: principles, advances, and applications.
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DOI：
10.1016/j.copbio.2016.06.003
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发表时间：
2016-08
影响因子：
7.7
通讯作者：
Hasty J
中科院分区：
文献类型：
--
作者：
Didovyk A;Borek B;Tsimring L;Hasty J
CRISPR-Cas9最近成为一种有前途的多路基因组编辑以及表观基因组和转录组扰动系统。由于其特异性、易用性和高度模块化可编程性，它已被广泛应用于各种应用，如基因组编辑、转录抑制和激活、遗传筛选、DNA定位成像等。在这篇综述中，我们将讨论CRISPR-Cas9在转录组扰动、代谢工程和合成生物学方面的非编辑应用。
CRISPR-Cas9 has recently emerged as a promising system for multiplexed genome editing as well as epigenome and transcriptome perturbation. Due to its specificity, ease of use and highly modular programmable nature, it has been widely adopted for a variety of applications such as genome editing, transcriptional inhibition and activation, genetic screening, DNA localization imaging, and many more. In this review, we will discuss non-editing applications of CRISPR-Cas9 for transcriptome perturbation, metabolic engineering, and synthetic biology.