Bacterial community dynamics during the preferential degradation of aromatic hydrocarbons by a microbial consortium
Bacterial community dynamics during the preferential degradation of aromatic hydrocarbons by a microbial consortium
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DOI：
10.1016/j.ibiod.2012.04.022
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发表时间：
2012-10-01
影响因子：
4.8
通讯作者：
Inoue, Chihiro
中科院分区：
文献类型：
--
作者：
Bacosa, Hernando Pactao;Suto, Koichi;Inoue, Chihiro
本研究的目的是确定负责快速降解芳烃的细菌，并确定细菌物种在微生物群落中的作用。四个主要细菌群，即伯克霍尔德氏菌属，无色杆菌属/产碱菌属，贪铜菌属，和红杆菌属。在Y联合体中，使用定量实时聚合酶链反应（q-PCR）评估优先降解的石油衍生芳烃。对二甲苯用作代表性芳族化合物，癸烷用作代表性脂族化合物，对二甲苯-癸烷用作代表性烃混合物，煤油用作代表性石油基油。结果表明，随着时间的推移，伯克霍尔德氏菌的数量显著增加。与煤油中的芳烃以及单一和双基质培养物中的对二甲苯的快速降解同时发生。贪铜菌属当癸烷迅速耗尽时显著增加。无色杆菌属，/产碱菌属，和罗丹明菌属（Rhodanoptera spp.）最初被芳香烃的存在所抑制，并且似乎在烃降解中不活跃，但利用代谢产物。这项工作提供了一种替代的方式来解释不同的微生物物种在烃馏分的降解的贡献。该财团的行为是在石油产品污染的环境中的毒性更大的芳烃的生物修复的背景下的利益。(C)2012爱思唯尔有限公司保留所有权利。
The aims of this study were to identify the bacteria responsible for the rapid degradation of aromatic hydrocarbons, and to determine the roles of bacterial species in the microbial consortium. The population changes of the four major bacterial groups, namely Burkholderia spp., Achromobacter spp./Alcaligenes spp., Cupriavidus spp., and Rhodanobacter spp. in the Y consortium that preferentially degraded petroleum oil-derived aromatic hydrocarbons were assessed using quantitative real-time polymerase chain reaction (q-PCR). P-xylene was used as a representative aromatic compound, decane as a representative aliphatic compound, p-xylene-decane as a representative hydrocarbon mixture, and kerosene as a representative petroleum-based oil. The results showed that the remarkable increase of Burkholderia spp. coincided with the rapid degradation of aromatic hydrocarbons in kerosene, and p-xylene in single and dual-substrate cultures. Cupriavidus spp. significantly increased when decane was rapidly depleted. Achromobacter spp.,/Alcaligenes spp., and Rhodanobacter spp. were initially inhibited by the presence of aromatic hydrocarbons and seemed not to be active in hydrocarbon degradation, but utilized the metabolic products. This work provided an alternative way to explain the contribution of different microbial species in the degradation of hydrocarbon fractions. The behavior of the consortium is of interest in the context of the bioremediation of the more toxic aromatic hydrocarbons in environments contaminated by petroleum products. (C) 2012 Elsevier Ltd. All rights reserved.