One-step immunoassay of C-reactive protein using droplet microfluidics
One-step immunoassay of C-reactive protein using droplet microfluidics
复制标题
使用液滴微流控技术对 C 反应蛋白进行一步免疫测定
DOI：
10.1039/c6lc01121g
复制
发表时间：
2016-01-01
期刊：
影响因子：
6.1
通讯作者：
Shum, Ho Cheung
中科院分区：
文献类型：
--
作者：
Tang, Matthew Y. H.;Shum, Ho Cheung
我们提出了一种免清洗的高灵敏度免疫分析的C-反应蛋白与液滴微流体。将微珠包封在用于免疫测定的液滴内，并且通过荧光检测平台扫描液滴以定量微珠上捕获的蛋白质的量。我们的平台确定的检测限为0.01 μ g mL（-1），比传统的高灵敏度C反应蛋白检测灵敏十倍。随着液滴内扩散距离的减小，免疫测定仅需要类似常规方法所需时间的一半。这种用于进行免疫测定的方法可以潜在地应用于C反应蛋白以外的其他生物标志物。
We present a wash-free high-sensitivity immunoassay of C-reactive proteins with droplet microfluidics. Microbeads are encapsulated within droplets for the immunoassay, and the droplets are scanned by a fluorescence detection platform to quantify the amount of proteins captured on the microbeads. The limit of detection determined by our platform is 0.01 mu g mL(-1), which is ten times more sensitive than conventional high-sensitivity C-reactive protein assays. With the decrease in diffusion distance within droplets, the immunoassay requires only half of the time required for similar conventional approaches. This approach for carrying out immunoassays can potentially be applied to other biomarkers beyond C-reactive proteins.