Arginine methylation of the RGG box does not appear to regulate ICP27 import during herpes simplex virus infection.
Arginine methylation of the RGG box does not appear to regulate ICP27 import during herpes simplex virus infection.
复制标题
RGG 盒的精氨酸甲基化似乎不会在单纯疱疹病毒感染期间调节 ICP27 输入。
DOI：
10.1128/jvi.00679-11
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发表时间：
2011
影响因子：
5.4
通讯作者：
Sandri-Goldin,RozanneM
中科院分区：
文献类型：
--
作者：
Souki,StuartK;Hernandez,FeliciaP;Sandri-Goldin,RozanneM
精氨酸甲基化可以调节蛋白质的输入和输出，并且可以调节蛋白质相互作用。单纯疱疹病毒I型（HSV-1）ICP 27是参与病毒mRNA输出的穿梭蛋白。我们以前报道，ICP 27是甲基化的三个精氨酸在其RGG盒和精氨酸甲基化调节ICP 27的出口和SRPK 1和Aly/REF的相互作用。在这里，我们报告，ICP 27被有效地进口到细胞核时，低甲基化的测定bachelocenceRecoveryAfterPhotobleaching（FRAP）。此外，ICP 27与β-importin的免疫共沉淀不受ICP 27低甲基化的显著影响。因此，ICP 27的输入似乎不受精氨酸甲基化的调节。
Arginine methylation can regulate protein import and export and can modulate protein interactions. Herpes simplex virus 1 (HSV-1) ICP27 is a shuttling protein involved in viral mRNA export. We previously reported that ICP27 is methylated on three arginines within its RGG box and that arginine methylation regulates ICP27 export and its interaction with SRPK1 and Aly/REF. Here, we report that ICP27 was efficiently imported into the nucleus when hypomethylated as determined byFluorescenceRecoveryAfterPhotobleaching (FRAP). Furthermore, coimmunoprecipitation of ICP27 with β-importin was not significantly affected by ICP27 hypomethylation. Thus, ICP27 import does not appear to be regulated by arginine methylation.