Increased nuclear factor-κB p65 immunoreactivity following retinal ischemia and reperfusion injury in mice
Increased nuclear factor-κB p65 immunoreactivity following retinal ischemia and reperfusion injury in mice
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DOI：
10.1002/jnr.10548
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发表时间：
2003-04-01
影响因子：
4.2
通讯作者：
Tso, MOM
中科院分区：
文献类型：
--
作者：
Chen, YG;Zhang, C;Tso, MOM
核因子-κ B（nuclear factor-kappaB，NF-kappaB）是一种通用的转录因子，在中枢神经系统损伤中起重要作用。本研究探讨了小鼠视网膜缺血再灌注损伤后视网膜内层NF-κ B的表达。视网膜缺血引起的眼内压升高至120毫米汞柱60分钟。为了评估内部视网膜变性，内部视网膜厚度定量与图像分析系统。视网膜缺血后24小时内视网膜内层厚度增加，并归因于组织水肿，但在随后的7天内显著降低。用NF-κ B p65单克隆抗体对视网膜进行免疫组化，并与TUNEL标记相关。视网膜缺血6小时后，内核层和神经节细胞层的核p65免疫反应性增强，24小时达到峰值，NF-κ B p65免疫标记的增强与TUNEL标记平行。p65和TUNEL双标记显示p65和TUNEL标记在内核层和神经节细胞层的散在细胞中部分共定位。然而，一些p65阳性细胞是TUNEL阴性的，表明这些细胞可能在损伤中存活。NF-κ B p65免疫反应性与视网膜缺血和再灌注损伤后的视网膜变性相关。(C)2003 Wiley-Liss，Inc.
Nuclear factor-kappaB (NF-kappaB) is a universal transcription factor and has previously been demonstrated to play an important role in CNS injury. This study investigated the expression of NF-kappaB in the inner layers of the retina in mice after retinal ischemia and reperfusion injury. Retinal ischemia was induced by elevation of intraocular pressure to 120 mmHg for 60 min. To evaluate inner retinal degeneration, the inner retinal thickness was quantified with an image-analysis system. The inner retinal thickness increased in the initial 24 hr after retinal ischemia and was ascribed to tissue edema but was significantly decreased in the ensuing 7 days. Immunohistochemistry using NF-kappaB p65 monoclonal antibody was performed on the retina and was corelated with TUNEL labeling. Six hours after retinal ischemia, nuclear p65 immunoreactivity was increased in the inner nuclear and ganglion cell layers and reached a peak at 24 hr. The increased NF-kappaB p65 immunolabeling was parallel to the TUNEL labeling. Double labeling with p65 and TUNEL showed partial colocalization of p65 and TUNEL labeling in the scattered cells of the inner nuclear and ganglion cell layers. However, several p65-positive cells were TUNEL negative, suggesting that these cells might have survived the injury. The NF-kappaB p65 immunoreactivity was associated with retinal degeneration following retinal ischemia and reperfusion injury. (C) 2003 Wiley-Liss, Inc.