Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity
Occurrence of Livestock-Associated Methicillin-Resistant Staphylococcus aureus in Turkey and Broiler Barns and Contamination of Air and Soil Surfaces in Their Vicinity
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DOI：
10.1128/aem.03939-12
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发表时间：
2013-04-01
影响因子：
4.4
通讯作者：
Roesler, U.
中科院分区：
文献类型：
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作者：
Friese, A.;Schulz, J.;Roesler, U.
从家禽养殖场排放的微生物，特别是耐甲氧西林金黄色葡萄球菌（MRSA）等耐药细菌是公众关注的问题，其发生和相关性存在争议。到目前为止，关于这个问题的数据有限。在这项研究中，我们调查了德国先前检测MRSA阳性的家禽畜棚内外的牲畜相关（LA）-MRSA的发生情况。总共对五个火鸡和两个肉鸡育肥场分别进行了四次和三次调查。在一个育肥期的纵向研究中，从动物、畜棚内的动物环境（包括空气）和畜棚周围环境（如环境空气和距畜棚不同距离处的地面靴子拭子）收集样品。此外，在育肥期的最后三分之一，在五个火鸡和四个肉鸡农场的谷仓内进行了一次横断面研究。在横断面研究中，在大多数谷仓（9个中的7个，77.8%）的空气中检测到LA-MRSA，以及来自动物的许多样品中，肉鸡的检测水平为50%至54%，火鸡农场为62%至77%。在纵向研究中，在两个火鸡谷仓外的环境空气中以及许多（44.4%）火鸡和肉鸡农场顺风侧的地面上发现了LA-MRSA。在畜舍内外观察到相同的SPA型分离物。MRSA在家禽养殖场内的传播以及通过空气传播途径的排放似乎是可能的。
The emission of microorganisms, especially resistant bacteria such as methicillin-resistant Staphylococcus aureus (MRSA), from poultry farms is of public interest, and its occurrence and relevance are controversially discussed. So far, there are limited data on this issue. In this study, we investigated the occurrence of livestock-associated (LA)-MRSA inside and outside previously tested MRSA-positive poultry barns in Germany. In total, five turkey and two broiler fattening farms were investigated four and three times, respectively. In a longitudinal study during one fattening period, samples were collected from animals, the animals' environment inside the barn, including the air, and the barns' surroundings, such as ambient air and boot swabs of ground surfaces at different distances from the barn. Moreover, a cross-sectional study was carried out once inside the barns on five turkey and four broiler farms during the last third of the fatting period. In the cross-sectional study, LA-MRSA was detected in the air of most barns (7 of 9, 77.8%), as well as in many samples originating from animals, with detections levels of 50 to 54% in broiler and 62 to 77% in turkey farms. In the longitudinal study, LA-MRSA was found in the ambient air outside two turkey barns and on the ground surface on the downwind side of many (44.4%) turkey and broiler farms. The same spa types of isolates were observed inside and outside the barns. Transmission of MRSA within poultry farms, as well as emission via the airborne route, seems to be possible.