A study of oral morphine preference in inbred mouse strains
A study of oral morphine preference in inbred mouse strains
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DOI：
10.1097/00041444-199422000-00003
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发表时间：
1994-01-01
影响因子：
0.9
通讯作者：
Vogel, W. H.
中科院分区：
文献类型：
--
作者：
Berrettini, W. H.;Alexander, R.;Vogel, W. H.
与DBA/2 J小鼠相比，C57 BL/6 J小鼠在两瓶选择范例中显示出增加的口服吗啡消耗。为了确定这种C57吗啡偏好是否反映了吗啡的受体介导的、基于奖励的作用的差异（与药代动力学或味觉差异相反），进行了三个实验。与先前的两瓶选择实验一致，C57平均值（+- S.D.）吗啡消耗量为18 ± 3 mg/kg/天，而DBA小鼠消耗量为1.4 ± 1.2 mg/kg/天。腹腔注射纳洛酮可使C57小鼠吗啡消耗量减少50%（p lt 0.01），而DBA小鼠则无变化。两种菌株的对照瓶中液体消耗量均未发生变化。口服吗啡溶液15分钟和30分钟后，这两种菌株之间吗啡及其葡萄糖醛酸苷衍生物的血浆水平没有差异。C57小鼠在0.05-0.4 mg/ml的吗啡浓度下维持约20 mg/kg的每日吗啡摄入量。这些实验表明，C57和DBA小鼠之间口服吗啡偏好的差异代表了一种基于奖励的机制，该机制通过阿片受体介导。
C57BL/6J mice, in two-bottle choice paradigms, show increased oral morphine consumption, compared with DBA/2J mice. To determine whether this C57 morphine preference reflects differences in the receptor-mediated, reward-based action of morphine (as opposed to pharmacokinetic or gustatory differences), three experiments were performed. Consistent with previous two-bottle choice experiments, C57 mean (+- S.D.) morphine consumption was 18 +- 3 mg/kg/day, while the DBA mice consumed 1.4 +- 1.2 mg/kg/day. Intraperitoneal naltrexone produced a 50% decrease in C57 morphine consumption (p lt 0.01), while DBA mice showed no change. Consumption of fluid from the control bottle was not changed for either strain. Fifteen and 30 min after oral consumption of a morphine solution, plasma levels of morphine and its glucuronide derivative were not different between these two strains. C57 mice maintained a daily morphine intake of approximately 20 mg/kg across morphine concentrations of 0.05-0.4 mg/ml. These experiments suggest that the difference in oral morphine preference between C57 and DBA mice represents a reward-based mechanism which is mediated through opiate receptors.