Evidence for expansion-based temporal BMP4/NOGGIN interactions in specifying periodontium morphogenesis
Evidence for expansion-based temporal BMP4/NOGGIN interactions in specifying periodontium morphogenesis
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DOI：
10.1007/s00441-007-0434-2
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发表时间：
2007-10-01
影响因子：
3.6
通讯作者：
Jung, Han-Sung
中科院分区：
文献类型：
--
作者：
Kim, Jae-Young;Cho, Sung-Won;Jung, Han-Sung
牙周组织中的牙囊细胞在小鼠牙周发育过程中具有分化为成纤维细胞、成牙骨质细胞和成骨细胞的能力。从胚胎第14天（E14）到出生后第11天（PN 11），组织学观察显示，牙周膜（PDL）形成区之间的牙槽骨形成和牙根形成区的相对宽度发生了显着变化。在PN 2，PDL形成区的宽度显示出最小值，但NOGGIN和增殖细胞核抗原的表达高于其他区域。在PN 11，PDL形成区域的相对宽度已经扩大。将发育中的牙胚的个别区域移植到肾被膜下，表明E14的牙囊细胞具有在3周后发育成矿化组织的潜力。这些结果表明，在PN 11 PDL宽度的恢复可能是由于细胞增殖和成骨因子及其拮抗剂之间的分子相互作用，如骨形态发生蛋白4（BMP 4）和NOGGIN之间的相互作用，类似于在缝合，肢体和体节形成中观察到的那些。为了证实BMP 4和NOGGIN之间的分子相互作用，在体外采用NOGGIN-蛋白质珠粒植入到培养物上。因此，这项研究表明，NOGGIN和BMP在PDL形成细胞中的和谐相互作用，表现出比邻近细胞更高的细胞增殖，可能对牙周组织的正常发育很重要。
Dental follicle cells in the periodontium are known to have the ability to differentiate into fibroblasts, cementoblasts, and osteoblasts during mouse periodontal development. From embryonic day 14 (E14) to postnatal day 11 (PN11), histological observations showed dramatic alterations in the relative width of the periodontal ligament (PDL)-forming region between the alveolar bone-forming and tooth root-forming area. At PN2, the width of the PDL-forming region showed a minimum, but with a higher expression of NOGGIN and proliferation cell nuclear antigen than the other regions. At PN11, the relative width of the PDL-forming region had expanded. Transplantation of individual regions of the developing tooth germ under the kidney renal capsule showed that dental follicle cells at E14 possessed the potential to develop into mineralized tissue after 3 weeks. These results suggested that the recovery of PDL width at PN11 may have resulted from cell proliferation and molecular interactions between osteogenic factors and their antagonists, such as interactions between bone morphogenetic protein 4 (BMP4) and NOGGIN, simlilar to those observed in suture, limb, and somite formation. To confirm the molecular interaction between BMP4 and NOGGIN, NOGGIN-protein bead implantation onto cultures was employed in vitro. This study thus indicates that harmonious interactions between NOGGIN and BMP in PDL-forming cells, which show higher cell proliferation than neighboring cells, might be important for proper periodontium development.