Identification of Mamu-DRB1 gene as a susceptibility factor for Entamoeba nuttalli infection in Chinese Macaca mulatta
Identification of Mamu-DRB1 gene as a susceptibility factor for Entamoeba nuttalli infection in Chinese Macaca mulatta
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Mamu-DRB1基因鉴定为中华猕猴内阿米巴感染易感因子
DOI：
10.1016/j.meegid.2021.104952
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发表时间：
2021
期刊：
影响因子：
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通讯作者：
Cheng Xunjia
中科院分区：
文献类型：
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作者：
Ling Zijian;Feng Meng;Xu Jian;Tachibana Hiroshi;Cheng Xunjia
纳氏内阿米巴感染在圈养和野生猕猴中高度流行。最近的研究表明，宿主猕猴的遗传因素与E.坚果分离物本研究旨在探讨恒河猴宿主主要组织相容性复合体基因与大肠杆菌的相关性。坚果感染从中国贵州省秦岭山采集了39份粪便样品。聚合酶链反应检测大肠杆菌感染率。大肠内阿米巴、查顿内阿米巴的检出率分别为69.23%、69.23%和87.18%。检测到一种新的富含丝氨酸蛋白基因型，并对E.黔灵山的菌株与GY 4菌株完全一致。在基于距离的邻接树中，Mamu-DRB 1基因与E.坚果感染黔灵山和龙湖山猕猴Mamu-DRB 1基因具有高度多态性，具有两种主要Mamu-DRB 1基因型的猕猴对E.坚果感染本研究对Mamu-DRB 1基因的分析表明，Mamu-DRB 1基因是影响大肠杆菌易感性的重要因素。本研究有助于更好地了解宿主对内阿米巴的易感性。
Entamoeba nuttalliinfection is highly prevalent in captive and wild macaques. A recent study suggested that the genetic factor of host macaques was correlated with the genotypes ofE. nuttalliisolates. This study focused on the correlation between the rhesus macaque host major histocompatibility complex gene andE. nuttalliinfection. Thirty-nine stool samples were obtained from Mount Qing-ling (Guizhou Province, China). Polymerase chain reaction analysis detected the infection rate ofE. nuttalli,Entamoeba coli, andEntamoeba chattonias 69.23%, 69.23%, and 87.18%, respectively. A new Serine-rich Protein genotype was detected, and the rRNA ofE. nuttalliisolates from Mount Qian-ling was completely identical to the GY4 strain. In the distance-based neighbor-joining tree,Mamu-DRB1, notMamu-DPBorMamu-Bgene, was related toE. nuttalliinfection. Mamu-DRB1genes of rhesus macaques in Mounts Qian-ling and Long-hu were highly polymorphic, and the rhesus macaques with two major types ofMamu-DRB1showed susceptibility toE. nuttalliinfection. TheMamu-DRB1gene analysis in this study indicated that theMamu-DRB1gene is an important factor that influences the susceptibility ofE. nuttalliinfection in ChineseMacaca mulatta.This study contributes to a better understanding of host susceptibility toEntamoeba.