Clinical significance of human kallikrein gene 6 messenger RNA expression in colorectal cancer
Clinical significance of human kallikrein gene 6 messenger RNA expression in colorectal cancer
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DOI：
10.1158/1078-0432.ccr-04-2281
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发表时间：
2005-04-15
影响因子：
11.5
通讯作者：
Mori, M
中科院分区：
文献类型：
--
作者：
Ogawa, K;Utsunomiya, T;Mori, M
目的：人激肽释放酶基因6（KLK 6）是人激肽释放酶基因家族的成员，并且最近的研究发现许多激肽释放酶在各种恶性肿瘤中改变了表达模式。本研究的目的是量化KLK 6在恶性和良性结直肠组织中的表达，并统计分析KLK 6的表达水平是否与结直肠癌患者的临床病理变量和预后相关。Experimental Designs：配对的结直肠癌组织样本和相应的非癌组织从63例结直肠癌患者接受手术切除。结果：63例胃癌组织中有57例（90%）KLK 6 mRNA过表达。癌组织中KLK 6 mRNA的平均表达水平显著高于癌旁组织（P < 0.0001）。KLK 6的表达与肿瘤浸润深度（P < 0.05）、肝转移（P < 0.05）和晚期杜克分期（P < 0.01）相关。此外，KLK 6高表达患者的精算总生存期明显低于KLK 6低表达患者（5年总生存率：分别为54%和73%，P < 0.05）。本研究结果表明，KLK 6 mRNA在大肠癌组织中的表达显著高于非癌组织，KLK 6 mRNA的高表达与大肠癌的浸润深度、肝转移、晚期杜克分期及不良预后有关。
Purpose: Human kallikrein gene 6 (KLK6) is a member of the human kallikrein gene family, and recent studies have found that many kallikreins have altered expression patterns in various malignancies. The purpose of the current study was to quantify the expression of KLK6 in malignant and benign colorectal tissues and to statistically analyze whether KLK6 expression levels correlate with clinicopathologic variables and prognosis in patients with colorectal cancer.Experimental Designs: Paired colorectal tissue samples from cancerous and corresponding noncancerous tissues were obtained from 63 patients with colorectal cancer who underwent surgical resection. Quantitative analyses of KLK6 mRNA expression were done using real-time quantitative reverse transcription-PCR.Results: KLK6 mRNA overexpression in cancerous tissues compared with normal counterparts was observed in 57 of 63 (90%) patients. The mean expression level of KLK6 mRNA in cancerous tissues was significantly higher than that in noncancerous tissues (P < 0.0001). Elevated KLK6 expression was significantly correlated with serosal invasion (P < 0.05), liver metastasis (P < 0.05), and advanced Duke's stage (P < 0.01). Furthermore, patients with high KLK6 expression had a significantly poorer actuarial overall survival than patients with low KLK6 expression (5-year overall survival rates: 54% and 73%, respectively, P < 0.05).Conclusions: The results of this study indicated that KLK6 mRNA expression was significantly higher in cancerous than in noncancerous colorectal tissues, and high expression of KLK6 mRNA correlated with serosal invasion, liver metastasis, advanced Duke's stage, and a poor prognosis for patients with colorectal cancer.