Development of an intact blood-brain barrier in brain tissue transplants is dependent on the site of transplantation
Development of an intact blood-brain barrier in brain tissue transplants is dependent on the site of transplantation
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DOI：
10.1016/0963-6897(95)02037-3
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发表时间：
1996-03-01
影响因子：
3.3
通讯作者：
Rose, GM
中科院分区：
文献类型：
--
作者：
Granholm, ACE;Curtis, M;Rose, GM
将胎儿隔前脑组织移植到大鼠眼前房或头侧海马结构，以评价移植部位对完整血脑屏障（BBB）发育的影响。在移植后1、2、3和4周，通过静脉注射台盼蓝，其是一种正常情况下不穿透BBB的活体染色剂，以及特异性针对大鼠BBB的抗体SMI 71。在眼内隔移植物中，移植后1周存在台盼蓝的显著渗漏，这与少数不含任何SMI 71免疫反应性的层粘连蛋白免疫反应性血管有关。然而，移植后2周，眼内移植物显示出广泛的表达SMI 71免疫反应性的薄壁血管丛，并且没有台盼蓝渗漏，因此，在眼内移植物中，在移植后2周已经出现了一定程度的BBB。另一方面，在颅内移植物中，在移植后3周可以看到台盼蓝渗漏，移植后4周，首次观察到具有SMI 71免疫反应性的密集血管丛，因此，在颅内移植物中台盼蓝不透性的发展比眼内移植物延迟1 - 2周，在成年大鼠中使用心理应激作为短暂破坏BBB的手段的对照实验显示，台盼蓝渗漏的增加与SMI 71免疫反应性的消失密切相关，总之，这些研究表明，移植部位可以影响神经组织移植物中完整BBB的发育。
Transplantation of fetal septal forebrain tissue was performed to the anterior chamber of the eye, or intracranially to the rostral hippocampal formation in rats, to evaluate the impact of transplantation site on the development of an intact blood-brain barrier (BBB), The tissue was studied at 1, 2, 3, and 4 wk following transplantation by means of intravenous injection of Trypan blue, which is a vital stain not normally penetrating the BBB, as well as with an antibody specifically directed against the rat BBB, SMI71, In the intraocular septal transplants, there was a significant leakage of Trypan blue 1 wk postgrafting, associated with a few laminin-immunoreactive blood vessels that did not contain any SMI71-immunoreactivity However, at 2 wk postgrafting, the intraocular grafts exhibited an extensive plexus of thin-walled blood vessels expressing SMI71 immunoreactivity and no Trypan blue leakage, Thus, it appeared that a BBB had developed to some degree by 2 wk postgrafting in oculo, In the intracranial grafts, on the other hand, Trypan blue leakage could be seen as long as 3 wk postgrafting, and a dense plexus of blood vessels with SMI71 immunoreactivity was first seen at 4 wk postgrafting, Thus, the development of Trypan blue impermeability was delayed with 1 to 2 wk in the intracranial versus the intraocular grafts, Control experiments using psychological stress in adult rats as a means to transiently disrupt the BBB revealed that an increase in Trypan blue leakage correlated well with the disappearance of SMI71 immunoreactivity, Taken together, these studies demonstrate that the site of transplantation can influence the development of an intact BBB in neural tissue grafts.