Characterization of diverse viral vector preparations, using a simple and rapid whole-virion dot-blot method.
Characterization of diverse viral vector preparations, using a simple and rapid whole-virion dot-blot method.
复制标题
使用简单快速的全病毒粒子斑点印迹方法表征多种病毒载体制剂。
作者：
D. Nelson;J. Wahlfors;L. Chen;M. Onodera;R. Morgan
许多不同的病毒已被用作基因转移载体，包括逆转录病毒、腺病毒、腺相关病毒（AAV）、单纯疱疹病毒、SV40病毒和甲病毒（Semliki Forest和Sindbis病毒）。这些载体表征中的主要限速和耗时步骤之一是确定病毒载体滴度的过程。此外，没有“通用”方法可用于快速估计病毒载体制剂的滴度和效用。我们在这里证明，上清液从不同类别的病毒载体，无论是RNA或DNA基因组，可以快速评估一个简单的病毒斑点杂交，而无需事先提取核酸。该系统可以在1天内提供可靠的病毒载体上清液物理滴度筛选。
A number of different viruses have been adapted as gene transfer vectors, including retroviruses, adenoviruses, adenoassociated viruses (AAVs), herpes simplex virus, SV40 viruses, and alphaviruses (both Semliki Forest and Sindbis viruses). One of the major rate-limiting and time-consuming steps in the characterization of these vectors is the process of determining the viral vector titers. In addition, there is no "universal" method that can be used to rapidly estimate the titer and the utility of viral vector preparations. We demonstrate here that supernatant from diverse classes of viral vectors, with either RNA or DNA genomes, can be rapidly evaluated by a simple virus dot-blot hybridization without prior extraction of nucleic acids. This system can provide a reliable screen for physical titer of viral vector supernatants in 1 day.
SV40作为体内有效的基因转移载体。
DOI：
--
发表时间：
1996
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Strayer,DS
通讯作者：
Strayer,DS
DOI：
10.1073/pnas.93.21.11371
发表时间：
1996-10-15
影响因子：
11.1
作者：
Frolov, I;Hoffman, TA;Rice, CM
通讯作者：
Rice, CM