An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis
An inverse correlation of human peripheral blood regulatory T cell frequency with the disease activity of ulcerative colitis
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DOI：
10.1007/s10620-006-3191-2
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发表时间：
2006-04-01
影响因子：
3.1
通讯作者：
Nawata, H
中科院分区：
文献类型：
--
作者：
Takahashi, M;Nakamura, K;Nawata, H
有证据表明CD4(+) CD25(+)调节性T细胞在抑制肠道炎症中发挥着至关重要的作用。然而，它们在抑制炎症性肠病中的作用尚未得到解决。我们检查了炎症性肠病中调节性 T 细胞的比例。首先，我们从健康人的外周血中分离出CD4(+) CD45RO(+) CD25(+) T细胞，结果表明这些细胞能够深度抑制T细胞增殖并大量表达FoxP3，表明它们是调节细胞。采用流式细胞术分析患者和健康对照者外周血CD4(+) T细胞中CD45RO(+) CD25(+)的比例。活动性溃疡性结肠炎中CD4(+)CD45RO(+)CD25(+)T细胞频率显着低于对照和非活动性溃疡性结肠炎。 CD4(+) CD45RO(+) CD25(+) T细胞频率与溃疡性结肠炎的临床和内镜严重程度呈负相关。这些结果表明调节性 T 细胞的缺乏与溃疡性结肠炎的进展有关。
Evidence suggests that CD4(+) CD25(+) regulatory T cells play a crucial role in the suppression of intestinal inflammation. However, their role in the suppression of inflammatory bowel disease has not yet been addressed. We examined the proportion of regulatory T cells in inflammatory bowel disease. First, we isolated CD4(+) CD45RO(+) CD25(+) T cells from the peripheral blood of healthy persons and showed that these cells suppressed T cell proliferation profoundly and expressed FoxP3 abundantly, revealing that they are regulatory cells. Then the proportion of CD45RO(+) CD25(+) in peripheral blood CD4(+) T cells was analyzed in patients and healthy controls by flow cytometry. CD4(+) CD45RO(+) CD25(+) T cell frequency was significantly lower in active ulcerative colitis than in the control and inactive ulcerative colitis. CD4(+) CD45RO(+) CD25(+) T cell frequency was inversely correlated with the clinical and endoscopic severity of ulcerative colitis. These results suggest that a deficiency of regulatory T cells is associated with the progression of ulcerative colitis.