PRODUCTION OF INTERLEUKINS IN HUMAN IMMUNODEFICIENCY VIRUS-1-REPLICATING LYMPH-NODES
PRODUCTION OF INTERLEUKINS IN HUMAN IMMUNODEFICIENCY VIRUS-1-REPLICATING LYMPH-NODES
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DOI：
10.1172/jci114678
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发表时间：
1990-07-01
影响因子：
15.9
通讯作者：
GALANAUD, P
中科院分区：
文献类型：
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作者：
EMILIE, D;PEUCHMAUR, M;GALANAUD, P
为了记录免疫系统和HIV复制细胞之间发生的体内相互作用，我们使用原位杂交分析了IL-1 β的产生，IL-6、IL-2和INF-γ。在8例HIV-1感染者的增生淋巴结中。大量IL-1 β。在鼻窦中检测到成簇的IL-6产生细胞。个别IL-1 β很少。滤泡间区和滤泡区有IL-6产生细胞。IL-2-和INF-γ-在所有淋巴结隔室中观察到产生细胞，并在生发中心选择性富集。IL-1 β-的量和分布，IL-6和IL-2的产生细胞在HIV淋巴结中与在6个HIV无关的增生淋巴结中发现的那些不同。相反，较高水平的INF-γ在HIV-1淋巴结中观察到产生。在HIV淋巴结的生发中心（而不是在非HIV生发中心）中积累的CD 8+细胞积极参与该INF-γ。生产INF-.gamma.合成细胞与含有HIV核心抗原和HIV RNA的细胞直接接触。因此，高IFN-γ。产生可能表征抗HIV T细胞免疫应答，可能有助于控制病毒传播以及卵泡溶解的发展。
To document the in vivo interactions occurring between the immune system and HIV replicating cells, we analyzed using in situ hybridization the production of IL-1.beta., IL-6, IL-2, and INF-.gamma. in eight hyperplastic lymph nodes from HIV-1 infected patients. Numerous IL-1.beta. and IL-6-producing cells associated in clusters were detected in sinuses. Few individual IL-1.beta. and IL-6-producing cells were present in interfollicular and follicular areas. IL-2- and INF-.gamma.-producing cells were observed in all lymph node compartments, with a selective enrichment in germinal centers. The amount and distribution of IL-1.beta.-, IL-6-, and IL-2-producing cells in HIV lymph nodes were different from those found in six HIV unrelated hyperplastic lymph nodes. In contrast, a higher level of INF-.gamma. production was observed in HIV-1 lymph nodes. The CD8+ cells that accumulate in germinal centers of HIV lymph nodes (and not in non-HIV germinal centers) were actively involved in this INF-.gamma. production. INF-.gamma. synthesizing cells were in direct contact with cells containing HIV core antigens and HIV RNA. Thus a high IFN-.gamma. production may characterize anti-HIV T cell immune response, potentially contributing to control of viral spreading as well as to the development of follicle lysis.