Comparing DNA methylation profiles in saliva and intestinal mucosa
Comparing DNA methylation profiles in saliva and intestinal mucosa
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DOI：
10.1186/s12864-019-5553-0
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发表时间：
2019-02-28
期刊：
影响因子：
4.4
通讯作者：
Lind, Joanne M.
中科院分区：
文献类型：
--
作者：
Hearn, Nerissa L.;Coleman, Aaron S.;Lind, Joanne M.
背景改变的表观遗传谱是肠道疾病的一个特征，包括溃疡性结肠炎和克罗恩病。这些疾病中的DNA甲基化研究利用了难以收集的肠粘膜组织或血液，特别是对于大规模的研究。唾液是表观遗传学研究的一个有吸引力的替代方案，因为它易于收集并提供高质量的甲基化谱。该研究的目的是确定唾液作为一种替代DNA甲基化研究肠道disorders.ResultsDNA甲基化在唾液和肠粘膜样本进行了比较个体（n=10）进行内镜下使用的Illumina Infinium甲基化450K微珠芯片阵列的效用。我们发现，DNA甲基化在个体内的组织类型之间存在相关性（Pearson相关系数r=0.92至0.95，p
BackgroundAltered epigenetic profiles are a feature of intestinal diseases, including ulcerative colitis and Crohn's disease. DNA methylation studies in these diseases have utilised intestinal mucosal tissue or blood which can be difficult to collect, particularly for large-scale research studies. Saliva is an attractive alternative for epigenetic studies as it is easy to collect and provides high quality methylation profiles. The aim of the study was to determine the utility of saliva as an alternative for DNA methylation studies of intestinal disorders.ResultsDNA methylation in saliva and intestinal mucosa samples were compared in individuals (n=10) undergoing endoscopies using the Illumina Infinium Methylation 450K Beadchip array. We found that DNA methylation was correlated between tissue types within an individual (Pearson correlation co-efficient r=0.92 to 0.95, p