Spatial Distribution of Putative Growth Factors in the Guinea Pig Placenta and the Effects of These Factors, Plasma, and Bile on the Growth and Chemotaxis of Campylobacter jejuni
Spatial Distribution of Putative Growth Factors in the Guinea Pig Placenta and the Effects of These Factors, Plasma, and Bile on the Growth and Chemotaxis of Campylobacter jejuni
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DOI：
10.1177/0300985811424755
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发表时间：
2012-05-01
影响因子：
2.4
通讯作者：
Yaeger, M. J.
中科院分区：
文献类型：
--
作者：
Burrough, E. R.;Wu, Z.;Yaeger, M. J.
妊娠豚鼠是研究致流产弯曲杆菌属的有效模型，已有实验表明，C. jejuni IA3902对胎盘下有明显的偏爱，而保留了胎盘盘。在本文所述的研究中，比较了IA3902和参考菌株（NCTC 11168）在妊娠和非妊娠豚鼠胎盘下和胎盘因子以及胆汁和血浆存在下的生长和趋化性。两种菌株在胎盘下和胎盘盘组织提取物中生长较好；然而，只有IA3902在48小时内保持了这种增强。组织化学和凝集素组织化学用于定位黏蛋白、铁和l-聚焦在胎盘单位内。粘蛋白在胎盘下腔隙、连接带和内脏卵黄囊胎盘中含量最多，铁在胎盘盘中含量最多，含l-焦的表面聚糖仅限于内脏卵黄囊胎盘。研究了这3种因子与黄体酮和雌二醇对空肠梭菌生长和趋化性的影响。粘蛋白、铁和l- focus对两种菌株都有促进生长的作用，而l- focus对两种菌株都有化学吸引作用。孕酮、雌二醇和妊娠豚鼠血浆不影响生长或趋化性，当比较妊娠动物和非妊娠动物的胆汁时，也没有观察到差异。这些发现表明，在豚鼠胎盘单元中存在驱动趋化性和促进空肠梭菌生长的特定因素，从而揭示了该菌株观察到的胎儿胎盘趋向性的潜在机制。
The pregnant guinea pig is an effective model for studying abortifacient Campylobacter spp, and previous experiments have demonstrated that C. jejuni IA3902 has a marked predilection for the subplacenta while sparing the placental disc in this species. In the study described here, the growth and chemotaxis of IA3902 and a reference strain (NCTC 11168) are compared in the presence of subplacental and placental factors, as well as bile and plasma, from pregnant and nonpregnant guinea pigs. Both strains grew better in subplacental versus placental disc tissue extracts at 24 hours; however, only IA3902 maintained this enhancement at 48 hours. Histochemistry and lectin histochemistry were used to localize mucin, iron, and l-fucose within the placental unit. Mucin was most abundant in subplacental lacunae, the junctional zone, and visceral yolk sac placenta, while iron was most abundant in the placental disc, and l-fucose-containing surface glycans were limited to the visceral yolk sac placenta. These 3 individual factors, along with progesterone and estradiol, were evaluated for effects on growth and chemotaxis of C. jejuni. Mucin, iron, and l-fucose were growth promoting, while l-fucose was also chemoattractive for both strains. Progesterone, estradiol, and pregnant guinea pig plasma did not affect growth or chemotaxis, and no difference was observed when bile from pregnant and nonpregnant animals was compared. These findings demonstrate the presence of specific factors within the guinea pig placental unit that drive chemotaxis and enhance growth of C. jejuni, shedding light on potential mechanisms underlying the fetoplacental tropism observed with this strain.