Identification and sequence analysis of two regulatory genes involved in anaerobic toluene metabolism by strain T1
Identification and sequence analysis of two regulatory genes involved in anaerobic toluene metabolism by strain T1
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DOI：
10.1128/aem.63.2.652-660.1997
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发表时间：
1997-02-01
影响因子：
4.4
通讯作者：
Young, LY
中科院分区：
文献类型：
--
作者：
Coschigano, PW;Young, LY
T1是一种能以甲苯作为唯一碳源生长的细菌，已分离出甲苯利用途径有缺陷的突变株，并根据生长表型将其分成几类，通过补充tutB 16分离出粘粒克隆（甲苯利用）突变，互补基因已定位于一个3.3-kb的DNA片段。tutB基因上游的另一个开放阅读框也已被鉴定并命名为tutC。T1菌株的tutB和tutC基因产物与双组分传感器-调节器家族的成员具有同源性，并被认为在T1菌株的甲苯代谢基因的调节中起作用。据我们所知，这是第一次发表的报告，分离缺陷的突变体在厌氧芳烃降解。此外，我们报告的第一次克隆的基因参与厌氧芳香烃降解途径。
T1 is a denitrifying bacterium isolated for its ability to grow with toluene serving as the sole carbon source, Mutants of this strain that have defects in the toluene utilization pathway have been isolated and have been separated into classes based on growth phenotypes, A cosmid clone has been isolated by complementing the tutB16 (for toluene utilization) mutation, The complementing gene has been localized to a 3.3-kb DNA fragment. An additional open reading frame upstream of the tutB gene has also been identified and is designated tutC. The nucleotide sequence and the predicted amino acid translation of the 6.4-kb DNA fragment that contains these genes are presented, The tutB and tutC gene products of strain T1 have homology to members of the two-component sensor-regulator family and are proposed to play a role in the regulation of toluene metabolic genes of strain T1. To our knowledge, this is the first published report of the isolation of mutants defective in anaerobic aromatic hydrocarbon degradation. Additionally, we report for the first time the cloning of genes involved in an anaerobic aromatic hydrocarbon degradation pathway.