Quantitation of Platelet and Fibrinogen‐Fibrin Deposition on Components of Tissue Valves (Ionescu‐Shiley) in Calves
Quantitation of Platelet and Fibrinogen‐Fibrin Deposition on Components of Tissue Valves (Ionescu‐Shiley) in Calves
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犊牛组织瓣膜 (Ionescu-Shiley) 组件上血小板和纤维蛋白原-纤维蛋白沉积的定量
DOI：
10.1097/00002480-198609000-00046
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发表时间：
1986
期刊：
影响因子：
4.2
通讯作者：
M. Kaye
中科院分区：
文献类型：
--
作者：
M. Dewanjee;E. Solis;J. Lenker;Caren Tidweli;S. Mackey;P. Didisheim;M. Kaye
用111 In标记的血小板和125 I标记的牛纤维蛋白原，获得了血小板和纤维蛋白原在瓣叶和缝合环上沉积的区域图。10头荷斯坦小牛植入25 mm二尖瓣（ISLM），并在植入后1、14和30天处死。在处死小牛前24小时，静脉注射350至450 microCi的111 In标记血小板和200至250 microCi的125 I标记牛纤维蛋白原。组织瓣膜的组成部分，即，分离了三个瓣叶和缝合环。将每个瓣叶切割成四个部分：自由边缘、中心区、屈曲区和附着区。根据血液中的放射性、瓣叶区、缝合环、瓣叶区面积、血小板计数和血液中的纤维蛋白原水平，计算粘附血小板、纤维蛋白原-纤维蛋白和纤维蛋白原/血小板的平均区域密度。瓣膜组件上沉积的血小板和纤维蛋白原密度随植入后时间的推移而降低。每个血小板的纤维蛋白原分子数量比瓣叶区血小板上的受体浓度高5倍至20倍，表明血栓和瓣膜组件中纤维蛋白原-纤维蛋白的异质性。
With 111In-labeled platelets and 125I-labeled bovine fibrinogen, regional mapping of platelet and fibrinogen deposition on leaflets and sewing rings was obtained. Ten Holstein calves received 25-mm mitral valves (ISLM) and were killed 1, 14, and 30 days after implantation. Twenty-four hours before the calves were killed, 350 to 450 microCi of 111In-labeled platelets and 200 to 250 microCi of 125I-labeled bovine fibrinogen were administered intravenously. The components of the tissue valves, i.e., three leaflets and sewing rings, were separated. Each leaflet was cut into four sections: free edge, central zone, flexion zone, and attachment zone. From the radioactivity in blood, leaflet zones, sewing rings, area of leaflet zones, platelet count, and fibrinogen level in blood, the mean regional density of adherent platelets, fibrinogen-fibrin, and fibrinogen/platelet were calculated. The density of platelets and fibrinogen deposited on the components of the valves decreases with time postimplantation. The number of fibrinogen molecules per platelet is fivefold to 20-fold higher than that of the receptor concentration on platelets on leaflet zones, suggesting the heterogeneity of fibrinogen-fibrin in thrombus and components of the valve.