Intensified expressions of a monocarboxylate transporter in consistently renewing tissues of the mouse
Intensified expressions of a monocarboxylate transporter in consistently renewing tissues of the mouse
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DOI：
10.2220/biomedres.32.293
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发表时间：
2011-08-01
影响因子：
1.2
通讯作者：
Iwanaga, Toshihiko
中科院分区：
文献类型：
--
作者：
Takebe, Kumiko;Takahasi-Iwanaga, Hiromi;Iwanaga, Toshihiko
在某些物理条件下，单羧酸-乳酸盐和酮体可以补偿葡萄糖作为能量来源。为了确定自我更新组织中使用的主要能量来源，参考葡萄糖转运蛋白，主要通过免疫组织化学方法研究了小鼠胃肠道、皮肤和骨髓中单羧酸转运蛋白（MCT）的表达谱。在小肠中，MCTI 免疫反应性上皮细胞在隐窝中积累，并沿着细胞基底外侧膜进行选择性免疫标记。 BrdU 标记的分裂细胞包含在隐窝 MCTI 免疫反应灶中。皮肤在毛发隆起处表现出强烈而广泛的 MCT1 免疫反应性，从而形成表皮、毛发和皮脂腺。食管的复层鳞状上皮基底层含有 MCT1 免疫反应细胞，但经常缺乏 GLUT1 免疫反应细胞。骨髓主要对 MCT1 产生免疫反应，但对 GLUT I 没有反应，表明在缺氧条件下单羧酸盐的活跃产生和利用用于造血。这些发现支持这样的观点：单羧酸盐是自我更新组织中最喜欢的能量来源。
Monocarboxylates-lactate and ketone bodies can compensate for glucose as energy sources under certain physical conditions. To identify the main energy source used in self-renewing tissues, expression profiles of monocarboxylate transporters (MCTs) were mainly investigated immunohistochemically in the gastrointestinal tract, skin, and bone marrow of mice, with reference to glucose transporters. In the small intestine, MCTI-immunoreactive epithelial cells accumulated in crypts with a selective immunolabeling along the basolateral membrane of cells. BrdU-labeled dividing cells were included in the cryptal MCTI-immunoreactive foci. The skin displayed an intense and extensive immunoreactivity for MCT1 in the hair bulge, which gives rise to the epidermis, hair, and sebaceous gland. The stratified squamous epithelium in the esophagus contained MCT1-immunoreactive cells in the basal layer but frequently lacked GLUT1-immunoreactive cells. The bone marrow was largely immunoreactive for MCT1 but not for GLUT I, suggesting the active production and utilization of monocarboxylates for hematopoiesis under hypoxic conditions. These findings support the idea that monocarboxylates are favorite energy sources in self-renewing tissues.