PURIFICATION AND ELECTRON-MICROSCOPY OF LACTIC-DEHYDROGENASE VIRUS OF MICE
PURIFICATION AND ELECTRON-MICROSCOPY OF LACTIC-DEHYDROGENASE VIRUS OF MICE
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DOI：
10.1099/0022-1317-26-3-217
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发表时间：
1975-01-01
影响因子：
3.8
通讯作者：
ELLENS, DJ
中科院分区：
文献类型：
--
作者：
HORZINEK, MC;VANWIELINK, PS;ELLENS, DJ
用琼脂糖凝胶过滤和等密度带沉降法从荷埃利希瘤小鼠感染性腹水中纯化乳酸脱氢酶病毒（LDV）。在甘油梯度中，确定病毒颗粒的沉降系数为约200 μ S，浮力密度为1.14 g/ml。球形颗粒，直径。根据固定和染色的方法，在62和80 nm之间的荧光已通过电子显微镜鉴定。病毒颗粒由包裹在双层包膜中的球形核衣壳组成。用NP 40处理分离并在蔗糖梯度上离心纯化的核衣壳具有176 S的沉降系数。电子显微镜照片显示球形颗粒，直径约为100 μ m。35 ± 4 nm。分类LDV作为披膜病毒科的成员进行了讨论。
Lactic dehydrogenase virus (LDV) was purified from infectious ascites fluid of mice bearing Ehrlich tumours using Sepharose gel filtration and rate zonal and isopycnic sedimentation. In glycerol gradients, a sedimentation coefficient of about 200S and a buoyant density of 1.14 g/ml was determined for the virus particle. Spherical particles with diam. between 62 and 80 nm, depending on the method of fixation and staining, have been identified electron microscopically. The virus particle consists of a spherical nucleocapsid wrapped into a double-layered envelope. The nucleocapsids, isolated by treatment with NP40 and purified by centrifuging on sucrose gradients had a sedimentation coefficient of 176S. Electron micrographs show spherical particles with a diam. of 35 ± 4 nm. Classification of LDV as a member of the togaviridae family is discussed.