Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes
Genetic diversity of avian paramyxovirus type 1: Proposal for a unified nomenclature and classification system of Newcastle disease virus genotypes
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DOI：
10.1016/j.meegid.2012.07.012
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发表时间：
2012-12-01
影响因子：
3.2
通讯作者：
Afonso, Claudio L.
中科院分区：
文献类型：
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作者：
Diel, Diego G.;da Silva, Luciana H. A.;Afonso, Claudio L.
禽副粘病毒1型(APMV-1)或新城疫病毒(NDV)由一组不同的病毒组成，基因组为单链负义RNA。在历史上，两种系统同时用于将新城疫病毒分离株分类为谱系或基因类型，造成命名上的混乱和遗传组分配上的差异。在本研究中，我们评估了禽副粘病毒1型(APMV-1)的遗传多样性，并提出了一个统一的命名和基于客观标准的分类系统来区分新城疫病毒的基因型别。I类(n=110)和II类(n=602)病毒的F基因全序列用于系统发育重建和鉴定不同的分类群。估计种群间的平均进化距离(10%)，并将其设置为分配新基因型别的分界值。我们的研究结果表明，I类病毒由单一的基因组成，而II类病毒包含15个基因组，其中包括10个已建立的基因组(I-IX和XI)和5个新的基因类型(X、XII、XIII、XIV和XV)。在I类和II类基因中可鉴定出亚型。采用统一的命名和客观标准对新城疫病毒分离株进行分类，将有助于研究新城疫病毒的流行病学、进化、疾病控制和诊断。爱思唯尔出版公司(Elsevier B.V.)
The avian paramyxovirus type 1 (APMV-1), or Newcastle disease virus (NDV), comprise a diverse group of viruses with a single-stranded, negative-sense RNA genome. Historically, two systems have been simultaneously used to classify NOV isolates into lineages or genotypes, generating confusion in the nomenclature and discrepancies in the assignment of genetic groups. In the present study we assessed the genetic diversity of the avian paramyxovirus type-1 (APMV-1) and propose a unified nomenclature and a classification system based on objective criteria to separate NDV into genotypes. Complete F gene sequences of class I (n = 110) and class II (n = 602) viruses were used for the phylogenetic reconstruction and to identify distinct taxonomic groups. The mean interpopulational evolutionary distance was estimated (10%) and set as the cutoff value to assign new genotypes. Results of our study revealed that class I viruses comprise a single genotype, while class II contains 15 genetic groups including 10 previously established (I-IX, and XI) and five new genotypes (X, XII, XIII, XIV and XV). Sub-genotypes were identified among class I and class II genotypes. Adoption of a unified nomenclature and of objective criteria to classify NDV isolates will facilitate studies on NOV epidemiology, evolution, disease control and diagnostics. Published by Elsevier B.V.