Urinary oligosaccharides of fucosidosis. Evidence of the occurrence of X-antigenic determinant in serum-type sugar chains of glycoproteins.
Urinary oligosaccharides of fucosidosis. Evidence of the occurrence of X-antigenic determinant in serum-type sugar chains of glycoproteins.
复制标题
岩藻糖苷沉积症尿寡糖。
DOI：
10.1093/oxfordjournals.jbchem.a132194
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发表时间：
1978
影响因子：
2.7
通讯作者：
A. Kobata
中科院分区：
文献类型：
--
作者：
M. Nishigaki;K. Yamashita;I. Matsuda;S. Arashima;A. Kobata
岩藻糖苷病患者的尿液中含有大量的岩藻糖基寡糖和富含岩藻糖的糖肽。通过Bio-Gel P-2和P-4柱层析和纸层析的组合纯化六种主要寡糖。通过连续外切糖苷酶消化和甲基化分析进行的结构研究表明，它们的结构如下：Fucalpha 1导致6 GlcNAc，Fucalpha 1导致2Galbeta 1导致4（Fucalpha 1导致3）GlcNAc β 1导致2 Manalpha 1导致3 Manbeta 1导致4GlcNAc，Galbeta 1导致4（Fucalpha 1导致3）GlcNAc beta1导致4 Manalpha 1导致4 GlcNAc，Galbeta 1导致4（Fucalpha 1导致3）GlcNAc β 1导致2 Manalpha 1导致6 Manbeta 1导致4GlcNAc，Galbeta 1导致4（Fucalpha 1导致3）GlcNAc beta1导致4 Manalpha 1导致6 Manalpha 1导致6 Manbeta 1导致4GlcNAc。此外，发现次要十糖的结构是Galbeta 1导致（Fucalpha 1导致）GlcNAcbeta 1导致Manalpha 1导致[Galbeta 1导致（Fucalpha 1导致）GlcNAcbeta 1导致Manalpha 1导致] Manbeta 1导致4GlcNAc。
Urine of a fucosidosis patient contained a large amount of fucosyl oligosaccharides and fucose-rich glycopeptides. Six major oligosaccharides were purified by a combination of Bio-Gel P-2 and P-4 column chromatographies and paper chromatography. Structural studies by sequential exoglycosidase digestion and by methylation analysis revealed that their structures were as follows: Fucalpha1 leads to 6GlcNAc, Fucalpha1 leads to 2Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 2Manalpha1 leads to 3Manbeta1 leads to 4GlcNAc, Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 4Manalpha1 leads to 4GlcNAc, Galbeta1 leads to 4(Fucalpha1 leads to3)GlcNAcbeta1 leads to 2Manalpha1 leads to 6Manbeta1 leads to 4GlcNAc, and Galbeta1 leads to 4(Fucalpha1 leads to 3)GlcNAcbeta1 leads to 4Manalpha1 leads to 6Manalpha1 leads to 6Manbeta1 leads to 4GlcNAc. In additon, the structure of a minor decasaccharide was found to be Galbeta1 leads to (Fucalpha1 leads to)GlcNAcbeta1 leads to Manalpha1 leads to [Galbeta1 leads to (Fucalpha1 leads to)GlcNAcbeta1 leads to Manalpha1 leads to]Manbeta1 leads to 4GlcNAc.