Protoplast Swelling and Hypocotyl Growth Depend on Different Auxin Signaling Pathways
Protoplast Swelling and Hypocotyl Growth Depend on Different Auxin Signaling Pathways
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DOI：
10.1104/pp.17.00733
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发表时间：
2017-10-01
期刊：
影响因子：
7.4
通讯作者：
Steffens, Bianka
中科院分区：
文献类型：
--
作者：
Dahlke, Renate I.;Fraas, Simon;Steffens, Bianka
运输抑制剂反应1/生长素信号F盒蛋白（TIR 1/AFB）家族的成员是已知的生长素受体。为了分析生长素结合蛋白1（ABP 1）的可能受体功能，我们采用了不同的方法。我们使用α-（2，4-二甲基苯乙基-2-氧代）-吲哚-3-乙酸（生长素）、α-（苯乙基-2-氧代）-吲哚-3-乙酸（PEO-IAA）和5-氟吲哚-3-乙酸（5-F-IAA）进行药理学方法以区分拟南芥（Arabidopsis thaliana）中ABP 1和TIR 1/AFB介导的过程。我们使用AtABP 1羧基末端区域的肽作为工具。我们用ABP 1、abp 1-c1和abp 1-TD 1的无效等位基因以及TILLING突变体abp 1 -5进行了突变体分析。我们采用了科英布拉，一种在生长素结合域的ABP 1表现出氨基酸交换的加入。我们测量无论是单下胚轴原生质体或下胚轴生长的体积变化，无论是在高时间分辨率。5-F-IAA选择性激活TIR 1/AFB途径，但不诱导原生质体膨胀;相反，它在下胚轴生长试验中显示生长素活性。与此相反，PEO-IAA诱导生长素样肿胀反应，但没有下胚轴生长。AtABP 1的羧基末端肽诱导生长素样的肿胀反应。在ABP 1相关突变体和科英布拉，没有生长素诱导的原生质体肿胀发生。ABP 1似乎参与介导快速生长素诱导的原生质体膨胀，但它不参与控制快速生长素诱导的生长。
Members of the TRANSPORT INHIBITOR RESPONSE1/AUXIN SIGNALING F-BOX PROTEIN (TIR1/AFB) family are known auxin receptors. To analyze the possible receptor function of AUXIN BINDING PROTEIN1 (ABP1), an auxin receptor currently under debate, we performed different approaches. We performed a pharmacological approach using alpha-(2,4-dimethylphenylethyl-2-oxo)-indole-3-acetic acid (auxinole), alpha-(phenylethyl-2-oxo)-indole-3-acetic acid (PEO-IAA), and 5-fluoroindole-3-acetic acid (5-F-IAA) to discriminate between ABP1- and TIR1/AFB-mediated processes in Arabidopsis (Arabidopsis thaliana). We used a peptide of the carboxyl-terminal region of AtABP1 as a tool. We performed mutant analysis with the null alleles of ABP1, abp1-c1 and abp1-TD1, and the TILLING mutant abp1-5. We employed Coimbra, an accession that exhibits an amino acid exchange in the auxin-binding domain of ABP1. We measured either volume changes of single hypocotyl protoplasts or hypocotyl growth, both at high temporal resolution. 5-F-IAA selectively activated the TIR1/AFB pathway but did not induce protoplast swelling; instead, it showed auxin activity in the hypocotyl growth test. In contrast, PEO-IAA induced an auxin-like swelling response but no hypocotyl growth. The carboxyl-terminal peptide of AtABP1 induced an auxin-like swelling response. In the ABP1-related mutants and Coimbra, no auxin-induced protoplast swelling occurred. ABP1 seems to be involved in mediating rapid auxin-induced protoplast swelling, but it is not involved in the control of rapid auxin-induced growth.