Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis
Cholesterol oxidase is required for virulence of Mycobacterium tuberculosis
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DOI：
10.1111/j.1574-6968.2007.00865.x
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发表时间：
2007-10-01
影响因子：
2.1
通讯作者：
Dziadek, Jaroslaw
中科院分区：
文献类型：
--
作者：
Brzostek, Anna;Dziadek, Bozena;Dziadek, Jaroslaw
最近的报告表明，胆固醇在巨噬细胞摄取分枝杆菌的过程中起着至关重要的作用。然而，由结核分枝杆菌编码的胆固醇修饰酶对细菌致病性的意义仍然未知。在这里，作者探讨了众所周知的胆固醇修饰酶，胆固醇氧化酶（ChoD），是否对结核杆菌的毒力很重要。利用同源重组技术替换M.结核病基因组具有无功能拷贝。所得突变体（Delta choD）在腹腔巨噬细胞中减弱。当用由热休克启动子（Delta choDP（hsp）choD）控制的完整choD基因补充相同菌株时，未观察到巨噬细胞中的衰减。小鼠感染实验证实了ChoD在M.结核
Recent reports have indicated that cholesterol plays a crucial role during the uptake of mycobacteria by macrophages. However, the significance of cholesterol modification enzymes encoded by Mycobacterium tuberculosis for bacterial pathogenicity remains unknown. Here, the authors explored whether the well-known cholesterol modification enzyme, cholesterol oxidase (ChoD), is important for virulence of the tubercle bacillus. Homologous recombination was used to replace the choD gene from the M. tuberculosis genome with a nonfunctional copy. The resultant mutant (Delta choD) was attenuated in peritoneal macrophages. No attenuation in macrophages was observed when the same strain was complemented with an intact choD gene controlled by a heat shock promoter (Delta choDP(hsp)choD). The mice infection experiments confirm the significance of ChoD in the pathogenesis of M. tuberculosis.