Frameshift suppression in Saccharomyces cerevisiae. V. Isolation and genetic properties of nongroup-specific suppressors.
Frameshift suppression in Saccharomyces cerevisiae. V. Isolation and genetic properties of nongroup-specific suppressors.
复制标题
酿酒酵母中的移码抑制。
DOI：
10.1093/genetics/102.3.361
复制
发表时间：
1982
期刊：
影响因子：
3.3
通讯作者：
Cummins,CM
中科院分区：
文献类型：
--
作者：
Culbertson,MR;Gaber,RF;Cummins,CM
在酿酒酵母（Saccharomycescerevisiae）中发现了分布于22个不同位点的两类移码抑制子。这些抑制子表现出等位基因特异性抑制甘氨酸或脯氨酸密码子中的+1 G：C插入突变，分别称为II组和III组移码突变。对应于每组代表性抑制子的基因已显示编码含有四个碱基反密码子的改变的甘氨酸或脯氨酸tRNA。该通讯报道了第三类移码抑制因子的存在，其抑制特异性范围更广。抑制基因定位于三个位点，suf12，suf13和suf14，分别位于染色体IV，XV和XIV。这些抑制因子的表型表明，抑制可能是由编码甘氨酸或脯氨酸tRNA的一级结构以外的基因介导的。
Two classes of frameshift suppressors distributed at 22 different loci were identified in previous studies in the yeastSaccharomyces cerevisiae. These suppressors exhibited allele-specific suppression of +1 G:C insertion mutations in either glycine or proline codons, designated as group II and group III frameshift mutations, respectively. Genes corresponding to representative suppressors of each group have been shown to encode altered glycine or proline tRNAs containing four base anticodons.—This communication reports the existence of a third class of frameshift suppressor that exhibits a wider range in specificity of suppression. The suppressors map at three loci,suf12, suf13, andsuf14, which are located on chromosomesIV, XV, andXIV, respectively. The phenotypes of these suppressors suggest that suppression may be mediated by genes other than those encoding the primary structure of glycine or proline tRNAs.