Quantitative phase imaging using a deep UV LED source
Quantitative phase imaging using a deep UV LED source
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DOI：
10.1364/ol.39.003468
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发表时间：
2014-06-15
期刊：
影响因子：
3.6
通讯作者：
Osten, Wolfgang
中科院分区：
文献类型：
--
作者：
Singh, Alok Kumar;Faridian, Ahmad;Osten, Wolfgang
我们提出了一种使用非相干深紫外（DUV）LED光源对生物和非生物样品进行高分辨率相位成像的方法。在不同的轴向平面记录物波的衍射图案，并且通过传播角谱来恢复相位。为了保持足够的光强度，我们避免使用针孔或光谱滤波器来增加DUV LED光源的相干性。这使得设置非常简单且具有成本效益。DUV光的短波长（285 nm），调谐到生物样品的吸收峰，允许同时获得高分辨率和高对比度图像。给出了实验结果来验证这一原理。(C)2014年美国光学学会
We propose a method for high resolution phase imaging of biological and non-biological samples using an incoherent deep ultraviolet (DUV) LED source. The diffraction pattern of the object wave is recorded at different axial planes and the phase is retrieved by propagation of the angular spectrum. To maintain enough light intensity, we avoided using a pinhole or spectral filter for increasing the coherence of the DUV LED source. This makes the setup very simple and cost effective. The short wavelength (285 nm) of the DUV light, tuned to the absorption peak of the biological samples, allows simultaneously high resolution and high contrast images. The experimental results are presented to verify this principle. (C) 2014 Optical Society of America