The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion
The silk gland proteome of Stenopsyche angustata provides insights into the underwater silk secretion
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DOI：
10.1111/imb.12874
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发表时间：
2023-09-23
影响因子：
2.6
通讯作者：
Dong，Zhao-Ming
中科院分区：
文献类型：
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作者：
Wang，Yu-Jun;Liu，Qing-Song;Dong，Zhao-Ming
卡迪斯虫（毛翅目）用粘胶丝编织各种水下复合结构。许多研究集中在球蚕丝的丝蛋白重链上，发现它含有重磷酸化以维持稳定的二级结构。除了纤维蛋白外，近年来的研究还发现了一些新的丝蛋白。为了更好地了解蛛丝的组成及其分泌过程，本研究报道了一种后退建筑线虫的丝腺蛋白质组，Stenopsyche angustataMartynov（毛翅目，线虫科）。采用液相色谱-串联质谱（LC‐MS/MS）技术，从蚕丝腺中鉴定出2389个蛋白。其中192种为分泌丝蛋白。在前丝腺中富集了29种蛋白，而在尾丝腺中富集了109种蛋白。丝素重链和9个未鉴定的丝蛋白被鉴定为磷酸化蛋白。通过分析丝素蛋白重链的序列，我们发现它包含13个Gly/Thr/Pro‐富区，12个Val/Ser/Arg‐富区和一个Gly/Arg/Thr‐富区。由于具有较大的分子量、信号肽和富含丝氨酸的重复基序，三种未鉴定的蛋白被鉴定为丝氨酸样蛋白。本研究为进一步阐明水下球蚕蚕丝的分泌和粘附提供了有价值的信息。
Caddisworms (Trichoptera) spin adhesive silks to construct a variety of underwater composite structures. Many studies have focused on the fibroin heavy chain of caddisworm silk and found that it contains heavy phosphorylation to maintain a stable secondary structure. Besides fibroins, recent studies have also identified some new silk proteins within caddisworm silk. To better understand the silk composition and its secretion process, this study reports the silk gland proteome of a retreat‐building caddisworm,Stenopsyche angustataMartynov (Trichoptera, Stenopsychidae). Using liquid chromatography tandem mass spectrometry (LC‐MS/MS), 2389 proteins were identified in the silk gland ofS. angustata, among which 192 were predicted as secreted silk proteins. Twenty‐nine proteins were found to be enriched in the front silk gland, whereas 109 proteins were enriched in the caudal silk gland. The fibroin heavy chain and nine uncharacterized silk proteins were identified as phosphorylated proteins. By analysing the sequence of the fibroin heavy chain, we found that it contains 13 Gly/Thr/Pro‐rich regions, 12 Val/Ser/Arg‐rich regions and a Gly/Arg/Thr‐rich region. Three uncharacterized proteins were identified as sericin‐like proteins due to their larger molecular weights, signal peptides and repetitive motifs rich in serine. This study provides valuable information for further clarifying the secretion and adhesion of underwater caddisworm silk.