A defect in carbohydrate metabolism ameliorates symptom severity in virus-infected Arabidopsis thaliana
A defect in carbohydrate metabolism ameliorates symptom severity in virus-infected Arabidopsis thaliana
复制标题
DOI：
10.1099/vir.0.82376-0
复制
发表时间：
2007-01-01
影响因子：
3.8
通讯作者：
Carr, John P.
中科院分区：
文献类型：
--
作者：
Handford, Michael G.;Carr, John P.
淀粉积累的改变是植物病毒感染的一种特征性生化症状。为了评估其生物学重要性，在连续光照（在此条件下没有淀粉的净分解）下生长的植物和缺乏叶绿体磷酸葡萄糖变位酶（淀粉生物合成所需的酶）的pgm 1突变体植物中研究了芜菁脉清病毒、黄瓜花叶病毒或花椰菜花叶病毒对拟南芥的感染。在连续光照下生长的病毒感染的野生型植物表现出更严重的叶片症状，但与在昼夜光照下生长的植物相比，生长没有减少。比较在永久光照下生长，pgm I突变体植物表现出比野生型对照更不严重的症状。然而，所有三种病毒的积累在野生型和植物中是相似的，并且不受光照条件的影响。结果表明，虽然在感染过程中淀粉积累的变化是不需要成功的病毒感染，碳水化合物代谢影响症状的发展。
Altered starch accumulation is a characteristic biochemical symptom of virus infection in plants. assess its biological importance, infection of Arabidopsis thaliana with Turnip vein-clearing virus, Cucumber mosaic virus or Cauliflower mosaic virus was investigated in plants grown under continuous illumination (under which there is no net breakdown of starch) and in pgm 1 mutant plants lacking chloroplastic phosphoglucomutase, an enzyme required for starch biosynthesis. Virus-infected wild-type plants grown under continuous light exhibited more severe leaf symptoms, but no reduction in growth compared with plants grown under diurnal illumination. Comparing grown in perpetual light, pgm I mutant plants displayed less severe symptoms than the wild-type controls. However, accumulation of all three viruses was similar in wild-type and plants and was unaffected by the light regime. The results show that, although changes in starch accumulation during infection are not required for successful viral infection, carbohydrate metabolism does influence symptom development.